Original scientific paper

https://doi.org/10.24099/vet.arhiv.0828

The influence of Theranekron® on glucose-6-phosphate dehydrogenase activity in rat ovaries, intact and with ischemia-reperfusion injury

Tolunay Kozlu ; Department of Histology and Embryology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey
Fatma Güler ; Institute of Health Sciences, Hatay Mustafa Kemal University, Hatay, Turkey
Pınar Peker Akalın ; Department of Biochemistry, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey
Filiz Kazak ; Department of Biochemistry, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey
Yaşar Ergün ; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey
Erdoğan Aslan ; Department of Obstetrics and Gynecology, Private Medicabil Hospital, Bursa, Turkey

Abstract

The effect of Theranekron® on rat ovaries was evaluated in healthy and ischemia-reperfusion injury models. The rats were divided into four groups: group 1: control, group 2: Theranekron® (single dose of 0.3 mg/kg intraperitoneally), group 3: torsion + detorsion, and group 4: torsion + detorsion + Theranekron® (single dose 0,3 mg/ kg, intraperitoneally). The ovaries were homogenized by sonication before the analysis and supernatant glucose-6- phosphate dehydrogenase activity, reduced nicotinamide adenine dinucleotide phosphate, reduced glutathione and total protein levels were evaluated spectrophotometrically. Ovary tissues were histologically examined. In group 2, glucose-6-phosphate dehydrogenase activity was significantly reduced (P<0.01) compared to the control group and an increased number of atretic follicles, and hyperaemic and haemorrhagic regions were seen histologically; in group 3, although glucose-6-phosphate dehydrogenase activity was not changed, intense hyperaemia and hemorrhage was observed in the veins of the medulla region and in the cortex. Group 4 showed a decrease in glucose-6-phosphate dehydrogenase activity compared to the control group (P<0.01). Histologically, when compared to group 3, group 4 showed fewer atretic follicles and decreased hyperaemia and hemorrhage in the ovaries, excluding the medulla region. There were no significant differences regarding reduced glutathione and reduced nicotinamide adenine dinucleotide phosphate levels between the groups. The Theranekron® dose applied had some negative effects, such as reduced glucose-6-phosphate dehydrogenase activity, an increased number of atretic follicles, and hyperemia in the corpus luteum and medulla region in intact rat ovaries. Although it reduced glucose-6-phosphate dehydrogenase activity, Theranekron® may have had slight remedial effects on rat ovaries with ischemia-reperfusion injury.

Keywords

Theranekron®; ovary; ischemia-reperfusion; glucose-6-phosphate dehydrogenase; rat

Hrčak ID:

255227

URI

https://hrcak.srce.hr/255227

Publication date:

6.4.2021.

Article data in other languages: croatian

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