Short communication, Note
Production of α-Amylase from Penicillium chrysogenum under Solid-State Fermentation by Using Some Agricultural By-Products
Bilal Balkan
; Trakya University, Science and Art Faculty, Department of Biology, TR-22 080 Edirne, Turkey
Figen Ertan
; Trakya University, Science and Art Faculty, Department of Biology, TR-22 080 Edirne, Turkey
Abstract
Solid-state fermentation (SSF) was carried out using corncob leaf (CL), rye straw (RS), wheat straw (WS) and wheat bran (WB) as substrates for α-amylase production by a fungal culture of Penicillium chrysogenum. The effects of moisture level, particle size and inoculum concentration on enzyme synthesis from P. chrysogenum were investigated. Optimal moisture levels of substrates were 75, 65, 65 and 55 % for CL, WS, WB and RS substrates, respectively. Optimal particle size and inoculum concentration for the production of α-amylase were: >1 mm, 20 %; >1 mm, 20 %; 1 mm, 20 % and >1 mm, 30 % for CL, WS, WB and RS, respectively. WB showed the highest enzyme production with 160 U/mL under optimum conditions. The other enzyme activities were 28, 49 and 45 U/mL using CL, RS and WS, respectively.
Keywords
α-amylase; Penicillium chrysogenum; solid-state fermentation
Hrčak ID:
23996
URI
Publication date:
13.12.2007.
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