Original scientific paper

https://doi.org/10.46419/vs.56.1.11

First report on the presence of potentially pathogenic Vibrio parahaemolyticus in farmed mussels (Mytilus galloprovincialis) from the Istrian aquatorium by microbiological and molecular methods

Natalija Džafić orcid.org/0000-0001-7658-5517 ; Hrvatski veterinarski institut - podružnica Veterinarski zavod Rijeka, Hrvatska
Barbara Boljkovac Begić orcid.org/0009-0000-1423-0075 ; Hrvatski veterinarski institut - podružnica Veterinarski zavod Rijeka, Hrvatska
Karmela Nina Barać ; Hrvatski veterinarski institut - podružnica Veterinarski zavod Rijeka, Hrvatska
Miroslav Benić ; Hrvatski veterinarski institut, Zagreb, Hrvatska
Sanja Duvnjak ; Hrvatki veterinarski institut, Zagreb, Hrvatska
Lidija Kozačinski ; Veterinarski fakultet Sveučilišta u Zagrebu, Hrvatska
Kristina Kvrgić orcid.org/0000-0002-2102-1113 ; Hrvatski veterinarski institut - podružnica Veterinarski zavod Rijeka, Hrvatska
Andrea Humski orcid.org/0000-0003-3027-1306 ; Hrvatski veterinarski institut, Zagreb, Hrvatska

Abstract

Microbiological and molecular PCR techniques were used to analyse 328 samples of mussels (Mytilus galloprovincialis) cultured in Istrian waters. Molecular testing was more effective than microbiological testing, detecting the presence of the bacterium Vibrio parahaemolyticus in 21.34% of samples, compared to 2.44% detected through microbiological methods. The probability of detecting the presence of this bacterium was 7.19 times higher when the sea surface temperature was above 15°C, while thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) positive samples were detected at sea temperatures above 22.5°C and 24.7°C, respectively. TDH- and TRH-positive samples constituted 10% of the total V. parahaemolyticus-positive samples. As there is no legal obligation to monitor the presence of this bacterium, consuming raw or insufficiently cooked mussels can lead to illness.

Keywords

mussels; Vibrio parahaemolyticus; microbiology; PCR

Hrčak ID:

317596

URI

https://hrcak.srce.hr/317596

Publication date:

11.6.2024.

Article data in other languages: croatian

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