Acta Pharmaceutica, Vol. 74 No. 3, 2024.
Original scientific paper
https://doi.org/10.2478/acph-2024-0026
Analysis of doxorubicin and fullerenol in rat serum by micellar electrokinetic capillary chromatography
RADE INJAC
; Faculty of Pharmacy, Chair of Pharmaceutical Biology, University of Ljubljana, 1000 Ljubljana, Slovenia
MIRANDA SERTIĆ
; Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Analysis, University of Zagreb, 10000 Zagreb, Croatia
NINA KOČEVAR GLAVAČ
; Faculty of Pharmacy, Chair of Pharmaceutical Biology, University of Ljubljana, 1000 Ljubljana, Slovenia
*
* Corresponding author.
Abstract
A new micellar electrokinetic capillary chromatographic (MEKC) method has been developed and optimized for simultaneous quantitation of doxorubicin (Dox) and fullerenol (Frl) in rat serum. The separation was carried out in a capillary (48.5–40 cm to the detector – 50 µm id fused-silica capillary with bubble cell, 150 µm) at an applied voltage of 25 kV and temperature of 25 °C. For the background electrolyte 10 mmol L–1 borate buffer pH 9.3 plus 15 mmol L–1 phosphate buffer pH 7.0 (with the final pH of the mixture adjusted to 7.0 with HCl), with added 10 % (V/V) methanol, and 15 mmol L–1 sodium dodecyl sulfate as a surfactant, were used. The hydrodynamic injection was carried out at 5.0 kPa during the period of 100 s. Linear calibration curves were established over the concentration range 0.5–500.0 mg L–1 for Dox and 10.0–500.0 mg L–1 for Frl (at 234 nm). The proposed MEKC procedure was fully validated and applied for the determination of Dox and Frl in Wistar rats after intraperitoneal administration of both molecules.
Keywords
fullerenol; doxorubicin; MEKC; rat serum
Hrčak ID:
317995
URI
Publication date:
30.9.2024.
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