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Chemistry in Industry : Journal of Chemists and Chemical Engineers of Croatia, Vol. 73 No. 13 (special issue), 2024.

Original scientific paper

https://doi.org/10.15255/KUI.2024.017

Optimisation of ASE for Determination of Organic Compounds Bound to Particulate Matter

Tajana Horvat orcid id orcid.org/0009-0002-8442-1189 ; Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10 000 Zagreb, Croatia
Ivana Jakovljević orcid id orcid.org/0000-0002-0556-0088 ; Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10 000 Zagreb, Croatia *
Zdravka Sever Štrukil orcid id orcid.org/0009-0006-5540-2332 ; Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10 000 Zagreb, Croatia
Gordana Pehnec orcid id orcid.org/0000-0001-5155-1847 ; Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10 000 Zagreb, Croatia

* Corresponding author.

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Abstract

Polycyclic aromatic hydrocarbons (PAHs), due to their harmful carcinogenic and mutagenic effects on human health, are significant organic pollutants in the air. Accelerated Solvent Extraction (ASE) at high temperature and pressure is a technique increasingly used to prepare samples for determining organic pollutants in environmental samples. To investigate ASE's efficacy in extracting organic pollutants bound to airborne particulate matter, model samples were prepared by spiking with known concentrations of a certified standard of PAHs. The following variables were optimised: solvent type, number of extraction cycles, extraction temperature, and flushing volume. ASE efficiency was compared with ultrasonic liquid extraction (ULE) using PAH certified reference material and real samples. All samples were analysed by high performance liquid chromatography with fluorescence detection and variable excitation and emission wavelength. Satisfactory efficiency for all PAHs were achieved using a solvent mixture of toluene and cyclohexane (7 : 3, v/v), two extraction cycles at 125 °C, and a flushing volume of 70 % of the cell volume, with recoveries exceeding 97 % for all PAHs except fluoranthene and pyrene (87 %).

Keywords

PAHs; liquid chromatography; benzo(a)pyrene; ultrasonic liquid extraction; human health

Hrčak ID:

322988

URI

https://hrcak.srce.hr/322988

Publication date:

12.12.2024.

Article data in other languages: croatian

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