Original scientific paper
https://doi.org/10.15644/asc59/3/1
Prevalence of Glutathione-S-Transferase T and M Deletion Polymorphisms in Apical Periodontitis: a Two-Center Observational Study
Mirsad Kadić
; Department of Dentistry, Sarajevo School of Science and Technology, Bosnia and Herzegovina; Faculty of Medicine, University of Banja Luka, Bosnia and Herzegovina
Nađa Nikolić
orcid.org/0000-0002-8513-9313
; Scientific Laboratories, Implant-Research Center, School of Dental Medicine, University of Belgrade, Serbia
Jelena Carkić
; Scientific Laboratories, Implant-Research Center, School of Dental Medicine, University of Belgrade, Serbia
Anesa Kadić-Pirović
; Faculty of Medicine, University of Banja Luka, Bosnia and Herzegovina
Katarina Beljić-Ivanović
; Department of Restorative Odontology and Endodontics, School of Dental Medicine, University of Belgrade, Serbia
Miroslav Andrić
orcid.org/0000-0003-4136-7901
; Department of Oral Surgery, School of Dental Medicine, University of Belgrade, Serbia
Naida Hadžiabdić
; Department of Oral Surgery, University of Sarajevo-Faculty of Dentistry with Dental Clinical Center, Bosnia and Herzegovina
Igor Đukić
; Department of Oral Surgery, Faculty of Medicine, University of Banja Luka, Bosnia and Herzegovina
Aleksandar Jakovljević
orcid.org/0000-0001-6512-4934
; Department of Pathophysiology, School of Dental Medicine, University of Belgrade, Serbia
Abstract
Objectives: The primary objective of this study was to examine the potential association between glutathione S-transferases (GSTT1/GSTM1) deletion polymorphisms and the development of apical periodontitis (AP) in a population of patients at two university centers: the Faculty of Medicine at the University of Banja Luka in Bosnia and Herzegovina and the School of Dental Medicine at the University of Belgrade in Serbia. Materials and Methods: The study involved 200 patients with AP in the experimental and 250 healthy individuals without AP in the control group. As a source of genomic DNA, sterile buccal swabs were taken from each patient. Genotyping of GSTM1 and GSTT1 deletion polymorphisms was conducted using multiplex Polymerase Chain Reaction (PCR). The risk of AP development with regard to the genotypes was evaluated based on odds ratios (ORs) and 95% confidence intervals (CIs) that were calculated via unconditional logistic regression. Results: There were significant differences in demographic characteristics between the investigated groups (p = 0.446, p = 0.154, respectively). GSTM1 and GSTT1 deletions were associated with a 3.05-fold and 5.69-fold risk (OR = 3.05, 95% CI = 2.07–4.49, OR = 5.69, 95% CI = 3.66–8.86, p < 0.001, p < 0.001, respectively) for the AP development. The co-occurrence of both deletions posed a significantly higher risk for AP development (OR = 52.76. 95% CI = 18.20–152.94, P < 0.001). Conclusions: The carriers of null GSTT, null GSTM, and double null GSTT/GSTM genotypes are more susceptible to AP development in the populations examined at the two centers.
Keywords
Periapical Periodontitis; Genetic Polymorphism; Glutathione S-Transferase; Apical periodontitis, Heredity, Oxidative stress, Deletion polymorphisms, Gluthathione-STransferase
Hrčak ID:
335350
URI
Publication date:
15.9.2025.
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