Veterinary Archives, Vol. 78 No. 6, 2008.
Original scientific paper
Influence of conjugated linoleic acid enriched ghee feeding on cancer incidence and histopathological changes in 7,12-dimethyl-benz[a]anthracene induced mammary gland carcinogenesis in rats
Kathirvelan Chinnadurai
; National Dairy Research Institute, Karnal, Haryana, India
Amrish Kumar Tyagi
; Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal, Haryana, India
Paramanandhan Krishnamoorthy
; Dr. ALM Postgraduate Institute of Basic Medical Sciences, Madras University, Chennai, Tamil Nadu, India
Abstract
A study was conducted to investigate the influence of Conjugated Linoleic Acid (CLA) feeding on cancer incidence and histopathological changes in DMBA induced mammary gland carcinogenesis rats. Female Wistar rats of 21 days were taken for the study and the animals were randomly divided into three groups of 30 animals each. Group I animals were fed with a soybean based diet, whereas group II and group III animals were fed with a low CLA and high CLA ghee based diet, respectively. Soybean oil, low CLA and high CLA ghee were included at the level of 20 per cent in the animal diet. Feeding with test diets started on the day of weaning. The animals were given 7,12 dimethyl-benz[a]anthracene(DMBA) at 5 mg per animal as a single dose by oral intubation at the age of 55 days and the test diets were continued up to 32 weeks after DMBA administration. At the end of 32 weeks all the animals were sacrificed, tumour data recorded and histopathology performed. The tumour incidence in groups I, II and III was 83.33, 63.33 and 46.07 per cent respectively. Histopathology revealed fibroma, adenoma, fibroadenoma in all the groups whereas in group I showed adenocarcinoma. Therefore, the results of the present study showed CLA not only inhibited benign type tumours but malignant tumours as well.
Keywords
mammary cancer; linoleic acid; dimethyl-benz[a]anthracene; histopathology
Hrčak ID:
31703
URI
Publication date:
18.12.2008.
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