Original scientific paper

Sinteza izoamilnog acetata pomoću lipaze u sustavu bez otapala s vinilnim acetatom kao donorom acila

Annapurna Kumari ; Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering, Department, Indian Institute of Technology, Kharagpur, IN-721302 West Bengal, India
Paramita Mahapatra ; Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering, Department, Indian Institute of Technology, Kharagpur, IN-721302 West Bengal, India
Vijay Kumar Garlapati ; Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering, Department, Indian Institute of Technology, Kharagpur, IN-721302 West Bengal, India
Rintu Banerjee ; Microbial Biotechnology and Downstream Processing Laboratory, Agricultural and Food Engineering, Department, Indian Institute of Technology, Kharagpur, IN-721302 West Bengal, India
Swagata Dasgupta ; Department of Chemistry, Indian Institute of Technology, Kharagpur, IN-721302 West Bengal, India

Abstract

Provedena je sinteza izoamilnog acetata, estera koji se često koristi kao pojačivač okusa u prehrambenoj industriji, u sustavu bez otapala. Pokušao se poboljšati prinos izoamilnog acetata transesterifikacijom s vinilnim acetatom pomoću imobilizirane lipaze izolirane iz soja Rhizopus oryzae NRRL 3562. Pri tome supstrat nije inhibirao imobiliziranu lipazu. Istražen je utjecaj različitih parametara reakcije na sintezu te ustanovljeno da je maksimalna pretvorba postignuta sa 16 % (m/V) imobilizirane lipaze pri 40 °C i 200 rpm. Pri tim je uvjetima nakon 8 sati postignuta 95 %-tna konverzija s 0,5 mol/L izoamilnog acetata. Struktura produkta utvrđena je infracrvenom spektroskopijom i nuklearnom magnetskom rezonancijom. Imobilizirana lipaza imala je Km vrijednost od 306,53 mmol/L i υmax od 99 µmol/(h∙g).

Keywords

izoamilni acetat; imobilizirana lipaza; Rhizopus oryzae NRRL 3562; sustav bez otapala; transesterifikacija; vinilni acetat

Hrčak ID:

33046

URI

https://hrcak.srce.hr/33046

Publication date:

26.3.2009.

Article data in other languages: english

Visits: 3.189 *