Original scientific paper

Enzymatic Hydrolysis of Yellowfin Tuna (Thunnus albacares) By-Products Using Protamex Protease

Huong Thi My Nguyen ; Nha Trang University, 2 Nguyen Dinh Chieu, Nha Trang, Vietnam
Khalifa Serigne Babacar Sylla ; IFREMER, rue de l'île d'Yeu, BP 21105, FR-44311 Nantes, France
Zo Randriamahatody ; IFREMER, rue de l'île d'Yeu, BP 21105, FR-44311 Nantes, France
Clair Donnay-Moreno ; IFREMER, rue de l'île d'Yeu, BP 21105, FR-44311 Nantes, France
Jacques Moreau ; ENSAT, avenue de l'Agrobiopole, BP 32607 Auzeville-Tolosane, FR-31326 Castanet-Tolosan cedex, France
Luyen Thi Tran ; Nha Trang University, 2 Nguyen Dinh Chieu, Nha Trang, Vietnam
Jean Pascal Bergé ; IFREMER, rue de l'île d'Yeu, BP 21105, FR-44311 Nantes, France

Abstract

Long-term proteolysis of tuna by-products (head, viscera and tail) by the wide spectrum protease Protamex has been investigated and compared. After hydrolysis, two fractions (soluble aqueous phase and insoluble sludge) were collected. Chemical compositions of each fraction and molecular mass distributions of soluble peptides were determined. Degrees of hydrolysis obtained after 12 h of hydrolysis of head, viscera and tail were 32.3, 16.8 and 22.2 %, respectively. Nitrogen recovery in the soluble fractions was 73.6 % for the head, 82.7 % for the viscera and 85.8 % for the tail. Lipid distribution indicated that the majority of lipids remained in the sludge. Such proteolysis appears useful for the production of very different fractions: one rich in peptides of medium to small molecular mass
and poor in lipids, and another one containing the insoluble proteins and the majority of lipids.

Keywords

tuna; by-products; proteolysis; nitrogen recovery; fish protein hydrolysates

Hrčak ID:

65525

URI

https://hrcak.srce.hr/65525

Publication date:

21.3.2011.

Article data in other languages: croatian

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