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Agriculturae Conspectus Scientificus, Vol. 75 No. 1, 2010.

Original scientific paper

Synergistic Effects of Combining Morphological and Molecular Data in Resolving the Intraspecific Classification in O. basilicum L.

Klaudija Carović- Stanko orcid id orcid.org/0000-0002-8777-9875 ; University of Zagreb, Faculty of Agriculture, Department of Seed Science and Technology, Svetošimunska 25, 10000 Zagreb, Croatia
Zlatko Liber ; University of Zagreb, Faculty of Science, Division of Biology, Department of Botany, Marulićev trg 9, 10000 Zagreb, Croatia
Martina Grdiša orcid id orcid.org/0000-0002-4584-4851 ; University of Zagreb, Faculty of Agriculture, Department of Seed Science and Technology, Svetošimunska 25, 10000 Zagreb, Croatia
Ivan Kolak ; University of Zagreb, Faculty of Agriculture, Department of Seed Science and Technology, Svetošimunska 25, 10000 Zagreb, Croatia
Zlatko Šatović orcid id orcid.org/0000-0002-9848-1601 ; University of Zagreb, Faculty of Agriculture, Department of Seed Science and Technology, Svetošimunska 25, 10000 Zagreb, Croatia

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Abstract

High levels of both morphological and chemical variability exist within the O. basilicum L. species. Long-term traditional uses and wide distribution throughout the world, as well as traditional selection and breeding efforts, have contributed to variability within the species. Morphological traits according to UPOV descriptor list and AFLP markers were utilized to define the extent of existing variation in the species analyzing 24 accessions. Phenotypic dissimilarities between pairs of accessions were calculated and the UPGMA dendrogram was constructed. A number of clearly defined clusters have been detected, giving a good representation of traditional taxonomic relationships. Genetic relationships were determined by Neighbour-Joining cluster analysis based on Dice’s distance matrix between accessions. Generally, morphologically similar accessions grouped together and a high congruence between trees was observed. Our analyses revealed a certain degree of correspondence between morphological and molecular data among O. basilicum L. accessions. Both AFLP markers and morphological descriptors can contribute in resolving existing problems concerning intraspecific classification in O. basilicum.

Keywords

AFLP; basil; morphological descriptors; UPOV

Hrčak ID:

53006

URI

https://hrcak.srce.hr/53006

Publication date:

26.3.2010.

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