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ANALYSIS OF ESTROGEN RECEPTOR POLYMORPHISM AND LITTER SIZE TRAITS IN PRIMIPAROUS SOWS: PRELIMINARY RESULTS
Sven Menčik
; Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Tomislav Balenović
; C. Zuzorić 1, Zagreb, Croatia
Slavko Lulić
; Krmiva Ltd., Zagreb, Croatia
Mario Modrić
; Krmiva Ltd., Zagreb, Croatia
Mario Ostović
; Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Velimir Sušić
; Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Igor Štoković
; Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Anamaria Ekert Kabalin
; Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
Identification of individual genes controlling litter size and their use in selection programs could contribute to an increased reproductive rate in pig population. Estrogen receptor gene (ESR) is a candidate gene marker for reproductive traits and growth in pigs. The aim of this preliminary study was to investigate ESR gene polymorphism as well as the frequencies of different ESR-PvuII genotypes and their litter size in primiparous sows. The polymerase chain reaction-restriction fragment
length polymorphism (PCR-RFLP) method with the PvuII restriction enzyme was used on genotyping 30 primiparous Topigs 20 hybrid sows. The following reproductive traits of litter size were analyzed: Total Number of Born piglets (TNB), Number of piglets Born Alive (NBA), Number of Stillborn piglets (NSB) and Number of Mummified piglets (NMUM). Allele and genotype frequencies were calculated, and Hardy-Weinberg equilibrium was tested using χ2-test. The ANOVA test was used to analyze litter size differences between sows with different genotypes. Three genotypes were detected with absolute frequencies (AA (13), AB (14) and BB (3)), while the gene relative frequency was 0.67 for A allele and 0.33 for B allele. Study animals were in Hardy-Weinberg equilibrium. The primiparous sows with the BB genotype
showed highest TNB (14.33) and NSB (2.33), while NBA and NMUM were highest in primiparous sows with AA genotype (12.66 and 0.38, respectively). Although some differences were found in litter size according to genotypes they were not significant (P>0.05). Further investigations in a larger sample size will contribute to more
conclusive evaluation of the possible correlation between ESR-PvuII polymorphism and litter size in sows.
Ključne riječi
estrogen receptor gene; Topigs 20; litter size
Hrčak ID:
101415
URI
Datum izdavanja:
25.4.2013.
Posjeta: 994 *