Original scientific paper
https://doi.org/10.17113/ftb.55.03.17.5040
Production of Hypoallergenic Antibacterial Peptides from Defatted Soybean Meal in Membrane Bioreactor: A Bioprocess Engineering Study with Comprehensive Product Characterization
Arij it Nath
; Department of Food Engineering, Faculty of Food Science, Szent István University, Ménesi st 44, HU-1118 Budapest, Hungary
Gábor Szécsi
; Department of Food Engineering, Faculty of Food Science, Szent István University, Ménesi st 44, HU-1118 Budapest, Hungary
Barbara Csehi
; Department of Refrigeration and Livestock Product Technology, Faculty of Food Science, Szent István University, Ménesi st 43–45, HU-1118 Budapest, Hungary
Zsuzsa Mednyánszky
; Department of Food Chemistry and Nutrition, Faculty of Food Science, Szent István University, Budapest, Somlói st 14–16, HU-1118 Budapest, Hungary
Gabriella Kiskó
; Department of Food Microbiology and Biotechnology, Faculty of Food Science, Szent István University, Budapest, Somlói st 14–16, HU-1118 Budapest, Hungary
Éva Bányai
; Department of Applied Chemistry, Faculty of Food Science, Szent István University, Budapest, Villányi st 29–33, HU-1118 Budapest, Hungary
Mihály Dernovics
; Department of Applied Chemistry, Faculty of Food Science, Szent István University, Budapest, Villányi st 29–33, HU-1118 Budapest, Hungary
András Koris
orcid.org/0000-0002-2284-8139
; Department of Food Engineering, Faculty of Food Science, Szent István University, Ménesi st 44, HU-1118 Budapest, Hungary
Abstract
Hypoallergenic antibacterial low-molecular-mass peptides were produced from defatted soybean meal in a membrane bioreactor. In the first step, soybean meal proteins were digested with trypsin in the bioreactor, operated in batch mode. For the tryptic digestion of soybean meal protein, optimum initial soybean meal concentration of 75 g/L, temperature of 40 °C and pH=9.0 were determined. After enzymatic digestion, low-molecular-mass peptides were purified with cross-flow flat sheet membrane (pore size 100 μm) and then with tubular ceramic ultrafiltration membrane (molecular mass cut-off 5 kDa). Effects of transmembrane pressure and the use of a static turbulence promoter to reduce the concentration polarization near the ultrafiltration membrane surface were examined and their positive effects were proven. For the filtration with ultrafiltration membrane, transmembrane pressure of 3·10^5 Pa with 3-stage discontinuous diafiltration was found optimal. The molecular mass distribution of purified peptides using ultrafiltration membrane was determined by a liquid chromatography–electrospray ionization quadrupole time-of-flight mass spectrometry setup. More than 96 % of the peptides (calculated as relative frequency) from the ultrafiltration membrane permeate had the molecular mass M≤1.7 kDa and the highest molecular mass was found to be 3.1 kDa. The decrease of allergenic property due to the tryptic digestion and membrane filtration was determined by an enzyme-linked immunosorbent assay and it was found to exceed 99.9 %. It was also found that the peptides purifi d in the ultrafi tration membrane promoted the growth of Pediococcus acidilactici HA6111-2 and they possessed antibacterial activity against Bacillus cereus.
Keywords
soybean meal; enzymatic hydrolysis; peptide separation; low-molecular-mass antibacterial peptides
Hrčak ID:
186541
URI
Publication date:
19.9.2017.
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