Izvorni znanstveni članak
https://doi.org/10.15567/mljekarstvo.2020.0403
The effect of in vitro digestion on antioxidant properties of water-soluble and insoluble protein fractions of traditional Serbian white- brined cheeses
Tanja Vučić
orcid.org/0000-0001-6492-9984
; University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
Danijel Milinčić
; University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
Slađana Žilić
; Maize Research Institute, Slobodana Bajića 1, 11081 Belgrade, Serbia
Ivana Ignjatović-Sredović
; University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
Zlatan Sarić
; University of Sarajevo, Faculty of Agriculture and Food Science, Zmaja of Bosne 8, 33000 Sarajevo, Bosnia and Herzegovina
Olivera Ećim-Đurić
; University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
Aleksandar Kostić
; University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
Miroljub Barać
; University of Belgrade, Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
Sažetak
The influence of simulated in vitro digestion on antioxidant potential of protein fractions of traditional Serbian white-brined cheeses was investigated. Water-soluble (WSF) and water-insoluble fractions (WINF) of three cow’s and three ovine white-brined cheeses were fractionated. Total antioxidant capacity, reducing power and iron (II) chelating properties of these fractions before and after in vitro digestion were assayed. The investigated protein fractions had different antioxidant properties. WSFs had a better total antioxidant capacity and reducing power and less pronounced iron (II) chelating properties than WINFs. A strong negative correlation (-0.818, P<0.05) between the total antioxidant capacities of undigested WSF and WINF of traditional cheeses were observed. In vitro digestion greatly improved the total antioxidant capacities of WINFs (by 16.61-34.18 times), their reducing power (up to 95.77 %) and except in the case of Svrljig ovine cheese, the iron (II) chelating ability as well. A less pronounced increase (up to 71.29 %) of the total antioxidant capacity of WSFs was induced by in vitro digestion. In vitro digestion reduced reducing power of WSF of investigated ovine cheeses as well as reducing power of WSF of Homolje cow’s cheese. Since there was no significant correlation between the investigated antioxidant properties of digested WSFs and their free amino acids and mineral contents the observed differences should be attributed to different composition and properties of low molecular weight peptides. Thus, further investigations related to their isolation and characterization needs to be conducted. However, these results indicate that Serbian white-brined cheeses have great potential as source of antioxidant peptides.
Ključne riječi
cheese; protein fractions; antioxidant properties; in vitro digestion
Hrčak ID:
243646
URI
Datum izdavanja:
14.9.2020.
Posjeta: 2.429 *