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SEPARATION AND DETERMINATION OF SULPHUR CONTAINING AMINO ACID ENANTIOMERS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Cs. Albert ; Hungarian University of Transylvania,Csíkszereda, Romania
K. Lóki ; University of Kaposvár, Faculty of Animal Science, Institute of Chemistry, H-7400 Kaposvár
É. Varga-Visi ; University of Kaposvár, Faculty of Animal Science, Institute of Chemistry, H-7400 Kaposvár
G. Pohn ; University of Kaposvár, Faculty of Animal Science, Institute of Chemistry, H-7400 Kaposvár
P. Sára ; University of Kaposvár, Faculty of Animal Science, Institute of Chemistry, H-7400 Kaposvár
J. Csapó ; University of Kaposvár, Faculty of Animal Science, Institute of Chemistry, H-7400 Kaposvár

Sažetak

Performic acid oxidation of cysteine and methionine resulting in the
formation of cysteic acid and methionine sulphon has been applied in order to avoid the loss of these two sulphur containing amino acids during the acidic hydrolysis of proteins that is necessary prior to amino acid analysis. The aim of the research was assigned by the increasing demand for the determination of the amount of amino acid enantiomers: the applicability of performic acid oxidation was evaluated from this point of view. Racemization of L-cysteine and L-methionine was found not significant during oxidation with performic acid, therefore this process can be applied before hydrolysis during quantification of cysteine and methionine enantiomers. Additionally, the quantification of cysteic acid and methionine sulphon enantiomers was accomplished in the form of their diastereoisomer derivatives via the development of a reversed phase of high performance liquid chromatography method.

Ključne riječi

performic acid oxidation; cysteine and methionine enantiomers; racemization

Hrčak ID:

23207

URI

https://hrcak.srce.hr/23207

Datum izdavanja:

16.10.2006.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.918 *