Izvorni znanstveni članak
https://doi.org/10.17508/CJFST.2024.16.1.01
Electrochemical characterization and detection of vitamin E in real samples
Dominik Goman
; Josip Juraj Strossmayer University of Osijek, Department of Chemistry
Valentina Danković
; Josip Juraj Strossmayer University of Osijek, Department of Chemistry
Olivera Galović
; Josip Juraj Strossmayer University of Osijek, Department of Chemistry
Martina Medvidović-Kosanović
; Josip Juraj Strossmayer University of Osijek, Department of Chemistry
Sažetak
Vitamin E is an important antioxidant and plays an essential role in cellular respiration. At room temperature, it exists in the form of viscous oil and is highly soluble in fat. Vitamin E is a group of eight compounds: four tocopherols and four tocotrienols. In this study, α-tocopherol (α -TOH) was characterized using cyclic and differential pulse voltammetry and was detected with high-performance liquid chromatography in real samples (kale, parsley, and a powdered dietary supplement). Cyclic and differential pulse voltammograms have shown one oxidation peak which corresponds to the oxidation of α -TOH. In the oxidation of α -TOH, rapid two-electron oxidation coupled with one proton loss \((-2e^{-}/-1H^{+})\) occurred, and a diamagnetic cation \((\alpha -TO^{+}) \) was formed. Oxidation of α -TOH α is an irreversible and a diffusion-controlled process since the linear correlation between anodic peak current, Ip,a, and the square root of scan rate \((v)^{1/2}\) was observed. It was also determined that the anodic peak current of α -TOH increases with the increase of its concentration (linear response was obtained in the concentration range from 0.43 \(mg/dm^{3} \) to 70.41 \(mg/dm^{3} \)). The obtained values of α -TOH in real samples were 8.267 mg/kg for parsley, 1.492 mg/kg for kale, and 0.067 mg/kg for powdered dietary supplement.
Ključne riječi
α –tocopherol; cyclic voltammetry; differential pulse voltammetry; high-performance liquid chromatography; vitamin E
Hrčak ID:
318160
URI
Datum izdavanja:
20.6.2024.
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