Combined in vitro effects of temozolomide and erucic acid on U87 MG cell viability, oxidative stress, and expression of genes involved in apoptotic signalling

Gecili, İbrahim; Genç, Sıdıka; Hacımüftüoğlu, Ahmet; Güner, Adem; Niğde, Esmanur; Karabulut, Kübra; Okan Yıllar, Dündar; Taghizadehehghalehjoughi, Ali; Abdelaty Hassibelnaby, Abdelaty Mostafa

doi:10.2478/aiht-2026-77-4098

Arhiv za higijenu rada i toksikologiju, Vol. 77 No. 2, 2026.

Izvorni znanstveni članak

https://doi.org/10.2478/aiht-2026-77-4098

Combined in vitro effects of temozolomide and erucic acid on U87 MG cell viability, oxidative stress, and expression of genes involved in apoptotic signalling

İbrahim Gecili ; Istanbul Beykent University Faculty of Medicine, Department of Medical Pharmacology, Istanbul, Turkey
Sıdıka Genç ; Bilecik Şeyh Edebali University Faculty of Medicine, Department of Medical Pharmacology, Bilecik, Turkey
Ahmet Hacımüftüoğlu ; Atatürk University Medical Faculty, Department of Medical Pharmacology, Erzurum, Turkey
Adem Güner ; Sinop University Faculty of Health Sciences, Department of Occupational Health and Safety, Sinop, Turkey
Esmanur Niğde ; Bilecik Şeyh Edebali University Faculty of Medicine, Department of Medical Pharmacology, Bilecik, Turkey
Kübra Karabulut ; Bilecik Şeyh Edebali University Faculty of Medicine, Department of Medical Pharmacology, Bilecik, Turkey
Dündar Okan Yıllar ; Istanbul Beykent University Faculty of Medicine, Department of Medical Pharmacology, Istanbul, Turkey
Ali Taghizadehehghalehjoughi ; Bilecik Şeyh Edebali University Faculty of Medicine, Department of Medical Pharmacology, Bilecik, Turkey
Abdelaty Mostafa Abdelaty Hassibelnaby ; Atatürk University Medical Faculty, Department of Medical Pharmacology, Erzurum, Turkey, 5 Cairo University Faculty of Veterinary Medicine, Department of Pharmacology, Giza, Egypt

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APA 6th Edition

Gecili, İ., Genç, S., Hacımüftüoğlu, A., Güner, A., Niğde, E., Karabulut, K., ... Abdelaty Hassibelnaby, A.M. (2026). Combined in vitro effects of temozolomide and erucic acid on U87 MG cell viability, oxidative stress, and expression of genes involved in apoptotic signalling. Arhiv za higijenu rada i toksikologiju, 77 (2), 123-130. https://doi.org/10.2478/aiht-2026-77-4098

MLA 8th Edition

Gecili, İbrahim, et al. "Combined in vitro effects of temozolomide and erucic acid on U87 MG cell viability, oxidative stress, and expression of genes involved in apoptotic signalling." Arhiv za higijenu rada i toksikologiju, vol. 77, br. 2, 2026, str. 123-130. https://doi.org/10.2478/aiht-2026-77-4098. Citirano 22.07.2026.

Chicago 17th Edition

Gecili, İbrahim, Sıdıka Genç, Ahmet Hacımüftüoğlu, Adem Güner, Esmanur Niğde, Kübra Karabulut, Dündar Okan Yıllar, Ali Taghizadehehghalehjoughi i Abdelaty Mostafa Abdelaty Hassibelnaby. "Combined in vitro effects of temozolomide and erucic acid on U87 MG cell viability, oxidative stress, and expression of genes involved in apoptotic signalling." Arhiv za higijenu rada i toksikologiju 77, br. 2 (2026): 123-130. https://doi.org/10.2478/aiht-2026-77-4098

Harvard

Gecili, İ., et al. (2026). 'Combined in vitro effects of temozolomide and erucic acid on U87 MG cell viability, oxidative stress, and expression of genes involved in apoptotic signalling', Arhiv za higijenu rada i toksikologiju, 77(2), str. 123-130. https://doi.org/10.2478/aiht-2026-77-4098

Vancouver

Gecili İ, Genç S, Hacımüftüoğlu A, Güner A, Niğde E, Karabulut K i sur. Combined in vitro effects of temozolomide and erucic acid on U87 MG cell viability, oxidative stress, and expression of genes involved in apoptotic signalling. Arh Hig Rada Toksikol. [Internet]. 2026 [pristupljeno 22.07.2026.];77(2):123-130. https://doi.org/10.2478/aiht-2026-77-4098

IEEE

İ. Gecili, et al., "Combined in vitro effects of temozolomide and erucic acid on U87 MG cell viability, oxidative stress, and expression of genes involved in apoptotic signalling", Arhiv za higijenu rada i toksikologiju, vol.77, br. 2, str. 123-130, 2026. [Online]. https://doi.org/10.2478/aiht-2026-77-4098

Sažetak

Limited efficacy of glioblastoma treatment with temozolomide (TMZ) due to resistance has prompted researchers to look for adjuvants capable of enhancing TMZ-associated cytotoxicity. The aim of our in vitro study was to evaluate such effects of erucic acid when combined with TMZ in human U87 MG cells. Cells were treated for 24 or 72 h with vehicle (control), TMZ (200 ng/mL), or TMZ plus EA (20, 40, 80, or 160 μg/mL). Cell viability was assessed using the MTT assay, membrane integrity with lactate dehydrogenase (LDH) release, and intracellular redox balance by measuring total antioxidant capacity (TAC) and total oxidant status (TOS). The mRNA expression of apoptosis-related genes (p53, p21, Bax, Bcl-2, Bcl-xL, and caspase-3) was analysed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Compared with the vehicle control, TMZ plus EA reduced cell viability and increased LDH release, with more pronounced effects at higher EA concentrations and at 72 h. These changes were accompanied by lower TAC and higher TOS, indicating a shift toward a pro-oxidant intracellular environment. At the transcriptional level, the combination induced increased pro-apoptotic Bax, caspase-3, p53, and p21 expression and reduced Bcl-2 and Bcl-xL expression. In conclusion, EA potentiated TMZ-associated cytotoxic responses in U87 MG cells with accompanying oxidative imbalance and apoptosis-related transcriptional changes.