Original scientific paper
The Use of Crude Shrimp Shell Powder for Chitinase Production by Serratia marcescens WF
Jesús E. Mejía-Saulés
; Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México DF, México
Krzysztof N. Waliszewski
; Instituto Tecnológico de Veracruz, M.A. de Quevedo 2779, P.O. Box 1380, Veracruz, 91860 México
Miguel A. Garcia
; Instituto Tecnológico de Veracruz, M.A. de Quevedo 2779, P.O. Box 1380, Veracruz, 91860 México
Ramon Cruz-Camarillo
; Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México DF, México
Abstract
From 102 Serratia marcescens strains screened, 57 strains showed chitinase activity and Serratia marcescens WF showed the highest chitinolytic activity so this strain was selected for further study on the use of crude shrimp waste for chitinase production. The concentration of crude shrimp shell content at 10–70 g/L, incubation temperature of 28–37 °C, pH=6–9, and time 24–96 h on kinetics of chitinase production by S. marcescens WF were evaluated. The maximal chitinase production related to process variables was obtained with the second order polynomial model: dry shrimp shell powder at 6 %, pH=6.5, temperature of 28 °C during fermentation for up to 72 h.
Keywords
crude shrimp waste; chitinase; Serratia marcescens WF; modeling
Hrčak ID:
109772
URI
Publication date:
15.3.2006.
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