Review article
Tetracycline antibiotics and determination of their residues in food
Sanja Makovec
; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Pierottijeva 6, Zagreb
Blaženka Kos
; Laboratorij za tehnologiju antibiotika, enzima, probiotika i starter kultura, Zavod za biokemijsko inženjerstvo, Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Pierottijeva 6, Zagreb
Jagoda Šušković
; Laboratorij za tehnologiju antibiotika, enzima, probiotika i starter kultura, Zavod za biokemijsko inženjerstvo, Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Pierottijeva 6, Zagreb
Nina Bilandžić
orcid.org/0000-0002-0009-5367
; Laboratorij za određivanje rezidua, Odjel za veterinarsko javno zdravstvo, Hrvatski veterinarski institut, Savska 143, Zagreb
Abstract
At the global level, almost 50% of the produced antibiotics are used in the prevention and therapies of domestic animals intended for human
consumption, and among them, tetracyclines are significant. Due to the broad spectrum of activity against Gram-positive and Gram-negative bacteria, rickettsia and viruses, relatively low toxicity, good tolerability and applications in various pharmaceutical forms, tetracycline antibiotics are widely used in human and veterinary medicine. Tetracyclines are products of secondary metabolism of bacteria of the genus Streptomyces, and are in use also as semisynthetic tetracyclines. Determination of antibiotic residues, including tetracyclines, in samples of animal origin, has become necessary in order to protect consumers and prevent the spread of antibiotic resistance. Control of antibiotic residues and maximum residue limits (MRLs) of antibiotics in certain organs, milk and tissues of animal origin are regulated at EU level. In order to control
tetracycline residues in food screening methods are used, such as various microbiological tests and ELISA methods. In case of positive results to tetracycline residues, above the prescribed maximum levels, confirmatory methods is applied, usually liquid chromatography with tandem mass spectrometry (LC-MS/MS).
Keywords
antibiotics; tetracyclines; antibiotic resistance; food of animal origin; food control
Hrčak ID:
126090
URI
Publication date:
31.7.2014.
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