Agronomy journal, Vol. 76 No. 1-2, 2014.
Original scientific paper
A RECORD OF POSTHARVEST BLACK ROT, ANTHRACNOSE AND GRAY MOULD ON MANDARIN FRUITS
Dario Ivić
; Hrvatski centar za poljoprivredu, hranu i selo, Zavod za zaštitu bilja, Zagreb, Hrvatska
Luka Popović
; Hrvatski centar za poljoprivredu, hranu i selo, Zavod za zaštitu bilja, Solin, Hrvatska
Ana Rogić
; Hrvatski centar za poljoprivredu, hranu i selo, Zavod za zaštitu bilja, Solin, Hrvatska
Mario Bjeliš
; Hrvatski centar za poljoprivredu, hranu i selo, Zavod za zaštitu bilja, Zagreb, Hrvatska
Abstract
Besides the dominant blue mould and green mould, caused by Penicillium species, the occurrence of other postharvest diseases of mandarins (Citrus unshiu) from Neretva Valley was noted during 2013. Thirty-seven fruits were sampled in packinghouses, and 37 isolates of Alternaria, Colletotrichum and Botrytis were collected. All 15 Colletotrichum isolates were identified as Colletotrichum gloeosporioides according to morphological features. All 16 Alternaria isolates were determined as Alternaria alternata, on the basis of morphology and polymerase chain reaction with AA-F2/AA-R3 primer pair. Remaining six isolates were identified as Botrytis cinerea. Three isolates of each fungal species were selected for pathogenicity test, performed by inoculation on the button end of unwounded fruits, inoculation on stylar end of the fruits and conidial suspension injection into the peel. C. Gloeosporioides isolates caused typical anthracnose symptoms after injection of conidia, but did not cause symptoms after button inoculation. B. cinerea isolates caused grey mould symptoms both after injection and button end inoculation. A. Alternata isolates did not cause symptoms after injection into the peel, but all isolates lead to development of typical black rot after button inoculation.
Keywords
Citrus unshiu; postharvest diseases; Alternaria; Colletotrichum; Botrytis
Hrčak ID:
129145
URI
Publication date:
17.10.2014.
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