Preliminary communication
https://doi.org/10.17113/ftb.53.02.15.3846
Isolation and Characterisation of Bacteriocin and Aggregation-Promoting Factor Production in Lactococcus lactis ssp. lactis BGBM50 Strain
Nemanja Mirkovic
; Faculty of Agriculture, University of Belgrade, Belgrade, Serbia
Zorica Radulovic
; Faculty of Agriculture, University of Belgrade, Belgrade, Serbia
Gordana Uzelac
; Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
Jelena Lozo
; Faculty of Biology, University of Belgrade, Belgrade, Serbia
Dragojlo Obradovic
; Faculty of Agriculture, University of Belgrade, Belgrade, Serbia
Ljubisa Topisirovic
; Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
Milan Kojic
; Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia
Abstract
Lactococcus lactis ssp. lactis BGBM50, a producer of lactococcin G and aggregation-promoting factor, was isolated from selected lactic acid bacteria taken from semi-hard cheese traditionally produced in the village Žanjic, Montenegro. Strain BGBM50 harbours a number of plasmids of diff erent sizes. Plasmid curing experiments showed that genes for bacteriocin production are located on pBM140, a plasmid 140 kb in length. PCR analysis with primers specifi c for lactococcin Q and G genes gave fragment of the expected size. In addition, after plasmid curing of strain BGBM50, different derivatives with altered phenotypes were obtained, among them BGBM50-34 strain, which retained bacteriocin synthesis but had enhanced aggregation ability.
Keywords
bacteriocin lactococcin G; aggregation-promoting factor; plasmid curing
Hrčak ID:
140240
URI
Publication date:
23.6.2015.
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