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Molecular method for identification of fallow venison

Nives Marušić Radovčić ; Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb
Ana Brekalo, dipl.ing. ; Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb
Tibor Janči ; Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb
Sanja Vidaček ; Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb
Helga Medić ; Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb
Prof. Goran Kušec ; Poljoprivredni fakultet Sveučilišta Josipa Juraja Strossmayera, Kralja Petra Svačića 1d, 31 000 Osijek

Abstract

Reliable and simple identification of species origin of meat and meat products can be carried out by applying species-specific primers that
target particular short DNA fragments. The aim of our research was to design species-specific primers for the amplification of mitochondrial
DNA control region of fallow deer (Dama dama) and to optimize the polymerase chain reaction protocol (PCR protocol) for such primers. Primers are used for the identification of fallow deer and the differentiation of fallow deer from other species, using only DNA extraction, PCR and electrophoresis. The application of primers was tested on eight fallow deer samples, three red deer and three roe deer samples.

Keywords

primer; mitochondrial DNA; fallow deer; Dama dama

Hrčak ID:

149286

URI

https://hrcak.srce.hr/149286

Publication date:

1.6.2015.

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