Original scientific paper

https://doi.org/10.17113/ftb.54.01.16.4135

Ascorbate Oxidase-Based Amperometric Biosensor for L-Ascorbic Acid Determination in Beverages

Gábor Csiffáry ; Eszterházy Károly College, EGERFOOD Knowledge Centre, Eger, Hungary
Péter Fűtő ; Eszterházy Károly College, EGERFOOD Knowledge Centre, Eger, Hungary
Nóra Adányi ; National Agricultural Research and Innovation Centre, Food Science Research Institute, Budapest, Hungary
Attila Kiss ; National Agricultural Research and Innovation Centre, Food Science Research Institute, Budapest, Hungary

Abstract

A novel biosensor for l-ascorbic acid determination in different beverages was elaborated. The ascorbate oxidase enzyme (AAO) from Cucurbita sp., EC 1.10.3.3, was immobilized on a screen-printed carbon electrode with poly(ethylene glycol) (400) diglycidyl ether (PEGDGE) as a crosslinking agent. The standards and samples were measured first with a blank electrode. An inert protein, bovine serum albumin (BSA), was immobilized on the surface of this electrode with PEGDGE. The BSA mass was equivalent to the mass of 10 U of AAO enzyme immobilized on the electrodes (0.021 mg). The linear measuring range for l-ascorbic acid was between 5 and 150 μmol/L. As l-ascorbic acid is a vital vitamin and a common antioxidant used in food industry, fruit juices and vitamin C effervescent tablets were examined. The results were compared to HPLC measurements.

Keywords

biosensor; l-ascorbic acid; ascorbate oxidase; screen-printed carbon electrodes

Hrčak ID:

155082

URI

https://hrcak.srce.hr/155082

Publication date:

31.3.2016.

Article data in other languages: croatian

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