Original scientific paper
https://doi.org/10.17113/ftb.54.04.16.4323
Assessment of the Antioxidant Activity of Silybum marianum Seed Extract and Its Protective Effect against DNA Oxidation, Protein Damage and Lipid Peroxidation
Aynur Serçe
; Chemistry Department, Science Faculty, Dicle University, TR–21280 Diyarbakır, Turkey
Bircan Çeken Toptancı
; Chemistry Department, Science Faculty, Dicle University, TR–21280 Diyarbakır, Turkey
Sevil Emen Tanrıkut
; Chemistry Department, Science Faculty, Dicle University, TR–21280 Diyarbakır, Turkey
Sevcan Altaş
; Chemistry Department, Science Faculty, Dicle University, TR–21280 Diyarbakır, Turkey
Göksel Kızıl
; Chemistry Department, Science Faculty, Dicle University, TR–21280 Diyarbakır, Turkey
Süleyman Kızıl
; Department of Field Crops, Faculty of Agriculture, Dicle University, TR–21280 Diyarbakır, Turkey
Murat Kızıl
orcid.org/0000-0003-2648-4915
; Chemistry Department, Science Faculty, Dicle University, TR–21280 Diyarbakır, Turkey
Abstract
Antioxidant properties of ethanol extract of Silybum marianum (milk thistle) seeds was investigated. We have also investigated the protein damage activated by oxidative Fenton reaction and its prevention by Silybum marianum seed extract. Antioxidant potential of Silybum marianum seed ethanol extract was measured using diff erent in vitro methods, such as lipid peroxidation, 1,1–diphenyl–2–picrylhydrazyl (DPPH) and ferric reducing power assays. The extract significantly decreased DNA damage caused by hydroxyl radicals. Protein damage induced by hydroxyl radicals was also effi ciently inhibited, which was confirmed by the presence of protein damage markers, such as protein carbonyl formation and by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE). The present study shows that milk thistle seeds have good DPPH free radical scavenging activity and can prevent
lipid peroxidation. Therefore, Silybum marianum can be used as potentially rich source of antioxidants and food preservatives. The results suggest that the seeds may have potential beneficial health effects providing opportunities to develop value-added products.
Keywords
Silybum marianum (milk thistle) seeds; antioxidant and radical scavenging activity, oxidative DNA damage; protein oxidation and lipid peroxidation; carbonyl formation; SDS–PAGE
Hrčak ID:
170000
URI
Publication date:
2.12.2016.
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