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Preliminary communication

https://doi.org/10.17113/ftb.56.01.18.5477

Proizvodnja pektinaze iz bakterije Bacillus sonorensis MPTD1

Anju Mohandas ; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST)
Sindhu Raveendran ; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST)
Binod Parameswaran ; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST)
Amith Abraham ; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST)
Raj S. R. Athira ; Academy of Scientific and Innovative Research (AcSIR)
Anil Kuruvilla Mathew ; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST)
Ashok Pandey ; CSIR-Indian Institute of Toxicology Research (CSIR-IITR)


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Abstract

U radu je ispitanaproizvodnja pektinaze na podlozi s agarom s pomoću sedam sojeva bakterija izoliranih iz pokvarenog voća i povrća. Najučinkovitiji soj, MPTD1, identificiran je kao soj bakterije Bacillus sonorensis. Primjenom Plackett-Burman i Box-Behnken statističkih planova optimirani su različiti parametri, te je utvrđeno da udjeliekstrakta kvasca, K2HPO4,NaNO3i KCl te vrijeme inkubacije negativno utječu na proizvodnju pektinaze. Najveća postignuta aktivnost enzima bila je 2,43 (μM/mL)/min. U ovom je radu po prvi put opisana proizvodnja pektinaze s pomoću bakterije Bacillus sonorensis.

Keywords

pektinaza; optimiranje fermentacije; Bacillus sonorensis

Hrčak ID:

197481

URI

https://hrcak.srce.hr/197481

Publication date:

30.3.2018.

Article data in other languages: english

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