Prethodno priopćenje
https://doi.org/10.17113/ftb.56.01.18.5477
Production of Pectinase from Bacillus sonorensis MPTD1
Anju Mohandas
; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST)
Sindhu Raveendran
; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST)
Binod Parameswaran
; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST)
Amith Abraham
; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST)
Raj S. R. Athira
; Academy of Scientific and Innovative Research (AcSIR)
Anil Kuruvilla Mathew
; Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST)
Ashok Pandey
; CSIR-Indian Institute of Toxicology Research (CSIR-IITR)
Sažetak
Seven isolates from spoiled fruits and vegetables were screened for pectinase produc¬tion using pectin agar plates and the most efficient bacterial strain, MPTD1, was identified as Bacillus sonorensis. Optimisation of various process parameters was done using Plack¬ett-Burman and Box-Behnken designs and it was found that parameters like yeast extract, K2HPO4, incubation time, NaNO3 and KCl have a negative impact on pectinase production. Parameters like pH and MgSO4 and pectin mass fractions have a positive impact on pecti¬nase production. The maximum obtained enzyme activity was 2.43 (μM/mL)/min. This is the first report on pectinase production by Bacillus sonorensis.
Ključne riječi
pectinase; fermentation optimization; Bacillus sonorensis
Hrčak ID:
197481
URI
Datum izdavanja:
30.3.2018.
Posjeta: 2.099 *