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Veterinarska stanica, Vol. 50 No. 6, 2019.

Original scientific paper

Prevalence and control of Listeria monocytogenes in food production

Maja Kiš orcid id orcid.org/0000-0002-4070-362X ; Veterinarski zavod Križevci, Hrvatski veterinarski institut, Hrvatska
Sanja Furmeg ; Veterinarski zavod Križevci, Hrvatski veterinarski institut, Hrvatska
Vesna Jaki Tkalec ; Veterinarski zavod Križevci, Hrvatski veterinarski institut, Hrvatska
Jadranka Sokolović ; Veterinarski zavod Križevci, Hrvatski veterinarski institut, Hrvatska
Krunoslav Sokolić ; MM Mesna industrija d.o.o., Krašić, Hrvatska
Željko Cvetnić ; Veterinarski zavod Križevci, Hrvatski veterinarski institut, Hrvatska

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Abstract

Listeria monocytogenes is a widespread bacterial species resistant to a wide range of unfavourable growth conditions, and therefore is considered to be one of the most important food-borne pathogens transmitted to humans. In this study, L. monocytogenes was detected in 28 (2.48%) of 1129 analysed food and environmental samples: 8 (3.48%) samples of meat and meat products, 4 (9.30%) samples of milk and dairy products, 2 (9.52%) samples of fruits, vegetables or fruit- or vegetable-based products, and in 14 (1.68%) environmental samples from food processing facilities. After conducting identification of growth colonies using primary identification tests, all isolates (28 samples) and 14 samples of bacterial cultures delivered for confirmation were confirmed for the presence of L. monocytogenes using the VITEK2 system and real-time PCR method. During routine microbiological analysis of food, pathogen detection is tested using the accepted standard protocols with the prescribed culture media. Fast methods for the detection of L. monocytogenes, such as real-time PCR, provide quick and reliable results, allowing food business operators to make a quick and efficient reaction in the case where contamination is detected during self- monitoring. The study results indicate that molecular methods, such as real-time PCR, are applicable for pathogen detection in different types of samples.

Keywords

Listeria monocytogenes; prevalence; food; real-time PCR

Hrčak ID:

228316

URI

https://hrcak.srce.hr/228316

Publication date:

21.11.2019.

Article data in other languages: croatian

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