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Acta Botanica Croatica, Vol. 79 No. 1, 2020.

Original scientific paper

https://doi.org/10.37427/botcro-2020-008

Somatic embryogenesis as a tool for virus elimination in Croatian indigenous grapevine cultivars

Nenad Malenica ; Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croatia
Mateja Jagić ; Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croatia
Bruno Pavletić ; Graduate Programme Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croatia
Nataša Bauer ; Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croatia
Darko Vončina ; Department of Plant Pathology, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, Zagreb, Croatia
Goran Zdunić ; Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, Split, Croatia
Dunja Leljak Levanić ; Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croatia

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Abstract

Most Croatian indigenous grapevine cultivars and vineyards are infected with a few dominant viruses. The goal of this study was to establish somatic embryogenesis as an efficient method for virus elimination from valuable Croatian cultivars and creating a reliable source of healthy plants. Somatic embryogenesis was induced from immature anthers and somatic embryogenesis-derived plantlets for seven indigenous cultivars were successfully regenerated. This procedure led to the elimination of viruses GFLV, GLRaV-1, GLRaV-3 and GFkV that were initially detected in the field-grown cultivars ‘Plavac mali’ and ‘Babica’ with an elimination success of at least 30%. The described method has the potential for production of virus-free rooted plantlets for all economically important cultivars or superior cultivar clones and for the establishment of a steady source of certified virus-free planting material.

Keywords

Babica; Croatian grapevine cultivars; plavac mali; RT-PCR; somatic embryogenesi; virus elimination; virus-free plants; Vitis vinifera L.

Hrčak ID:

233760

URI

https://hrcak.srce.hr/233760

Publication date:

1.4.2020.

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