Izvorni znanstveni članak

https://doi.org/10.37427/botcro-2020-008

Somatic embryogenesis as a tool for virus elimination in Croatian indigenous grapevine cultivars

Nenad Malenica ; Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croatia
Mateja Jagić ; Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croatia
Bruno Pavletić ; Graduate Programme Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croatia
Nataša Bauer ; Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croatia
Darko Vončina ; Department of Plant Pathology, Faculty of Agriculture, University of Zagreb, Svetošimunska 25, Zagreb, Croatia
Goran Zdunić ; Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, Split, Croatia
Dunja Leljak Levanić ; Division of MoleDivision of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croatiacular Biology, Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, Zagreb, Croati

Sažetak

Most Croatian indigenous grapevine cultivars and vineyards are infected with a few dominant viruses. The goal of this study was to establish somatic embryogenesis as an efficient method for virus elimination from valuable Croatian cultivars and creating a reliable source of healthy plants. Somatic embryogenesis was induced from immature anthers and somatic embryogenesis-derived plantlets for seven indigenous cultivars were successfully regenerated. This procedure led to the elimination of viruses GFLV, GLRaV-1, GLRaV-3 and GFkV that were initially detected in the field-grown cultivars ‘Plavac mali’ and ‘Babica’ with an elimination success of at least 30%. The described method has the potential for production of virus-free rooted plantlets for all economically important cultivars or superior cultivar clones and for the establishment of a steady source of certified virus-free planting material.

Ključne riječi

Babica; Croatian grapevine cultivars; plavac mali; RT-PCR; somatic embryogenesi; virus elimination; virus-free plants; Vitis vinifera L.

Hrčak ID:

233760

URI

https://hrcak.srce.hr/233760

Datum izdavanja:

1.4.2020.

Posjeta: 2.583 *