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Food Technology and Biotechnology, Vol. 47 No. 2, 2009.

Original scientific paper

Light-Dependent Betanin Production by Transformed Cells of Sugar Beet

Dubravko Pavoković orcid id orcid.org/0000-0001-7198-6063 ; Department of Molecular Biology, Division of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Gordana Rusak ; Department of Botany, Division of Biology, Faculty of Science, University of Zagreb, Marulićev trg 9a, HR-10000 Zagreb, Croatia
Višnja Besendorfer ; Department of Molecular Biology, Division of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Marijana Krsnik-Rasol ; Department of Molecular Biology, Division of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia

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Abstract

The objective of this work is to transform sugar beet (Beta vulgaris L. var. altissima) cells using Agrobacterium tumefaciens, a wild octopine strain B6S3, and to study metabolic changes associated with the transformation. From the primary tumours on leaf fragments, two tumour lines were established: one pale green and the other red-violet in colour. The red-violet pigment was identified by means of high-performance liquid chromatography as betanin. Betanin production is strongly light-dependent. To enhance the betanin yield, nutrient media with different carbohydrates like sucrose, a combination of glucose and fructose, or only glucose or only fructose were tested. The selection of carbohydrate affected betanin production and yield. It was observed that the production of betanin per g of dry mass was 20–40 % higher on simple carbohydrates, especially fructose, than on sucrose or the combination of glucose and fructose. However, due to higher biomass production on the medium with sucrose, the highest total yield of betanin was obtained in the presence of sucrose. The most suitable time for possible betanin extraction was between days 7 and 14 for tissue grown on sucrose. The tumour line seems to be promising as an alternative source of betanin as well as a model to study sugar-regulated genes involved in tissue morphology control.

Keywords

Agrobacterium tumefaciens; betacyanins; carbohydrates; in vitro tissue culture; pigment; secondary metabolites

Hrčak ID:

38106

URI

https://hrcak.srce.hr/38106

Publication date:

9.6.2009.

Article data in other languages: croatian

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