Izvorni znanstveni članak
Peripheral Whole Blood FOXP3 TSDR Methylation and Plasma IL-10, IL-17, IL-35, and IL-36 Levels in Psoriasis
Burcu Acikgoz
orcid.org/0000-0002-7245-7697
; Department of Molecular Medicine, Graduate School of Health Sciences, Dokuz Eylul University, Izmir, Turkey;
*
Ozlem Ozbagcivan
; Department of Dermatology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey
Sebnem Aktan
; Department of Dermatology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey
Serpil Tanriverdi Akhisaroglu
; Department of Molecular Medicine, Graduate School of Health Sciences, Dokuz Eylul University, Izmir, Turkey;
Harun Muayad Said
; Department of Molecular Medicine, Graduate School of Health Sciences, Dokuz Eylul University, Izmir, Turkey;
* Dopisni autor.
Sažetak
Recent studies have shown that methylation levels in regulator T-cells,
which are characterized by high FOXP3 expression, pro-inflammatory,
and anti-inflammatory cytokines are involved in the pathogenesis of
psoriasis. Our aim was to determine the levels of FOXP3 TSDR methyla-
tion, FOXP3 mRNA expression, and levels of IL-10, IL-17, IL-35, and IL-36
in patients with psoriasis. In addition, we intended to investigate the rela-
tionship of these parameters with disease severity and treatment status.
We performed a hospital-based case-control study on 38 patients with
psoriasis and 20 controls. We performed HRM-PCR, real-time PCR, and
ELISA to determine FOXP3 TSDR methylation status, FOXP3 mRNA ex-
pression, and interleukin levels, respectively. FOXP3 TSDR was found to
be methylated both in the patient and control groups. There was no sig-
nificant difference between the melting temperatures and FOXP3 mRNA
levels between the groups. Among the cytokines, only IL-10 was found to
be significantly lower in patients. Positive correlation was found between
IL-35 levels and psoriasis area and severity index (PASI) scores in all pa-
tients; IL-17A levels and PASI scores in patients with mild psoriasis, and IL-
36γ levels and PASI scores in patients with moderate-to-severe psoriasis.
The production of IL-10 seems to be impaired in patients with psoriasis.
Positive correlation between the levels of IL -35, IL-17A, and IL-36γ and
disease severity supports the clinical implication of these cytokines in
psoriasis.
Ključne riječi
psoriasis; regulator T-cell; FOXP3; methylation; interleukin
Hrčak ID:
334831
URI
Datum izdavanja:
20.6.2024.
Posjeta: 196 *