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Original scientific paper

„DNA barkodiranje“ za pouzdano utvrđivanje izvornosti morskih rakova, mekušaca i riba dostupnih na tržištu

Silvia Nicolè ; Laboratory of Genetics and Genomics, Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
Enrico Negrisolo ; Department of Comparative Biomedicine and Food Science, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
Giulia Eccher ; Laboratory of Genetics and Genomics, Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
Roberto Mantovani ; Laboratory of Genetics and Genomics, Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
Tomaso Patarnello ; Department of Comparative Biomedicine and Food Science, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
David L. Erickson ; Department of Botany and Laboratories of Analytical Biology, National Museum of Natural History, Smithsonian Institution, P.O. Box 37012, Washington, DC 20013-7012, USA
W. John Kress ; Department of Botany and Laboratories of Analytical Biology, National Museum of Natural History, Smithsonian Institution, P.O. Box 37012, Washington, DC 20013-7012, USA
Gianni Barcaccia orcid id orcid.org/0000-0001-7478-5048 ; Laboratory of Genetics and Genomics, Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy


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Abstract

DNA barkodiranje“ omogućuje istraživačima identifikaciju različitih životinjskih vrsta analizom kratke sekvencije nukleotida, i to obično mitohondrijskog gena cox1. U ovom je radu „DNA barkodiranje“ primjenjeno za identifikaciju uzoraka morskih rakova, mekušaca i riba kupljenih na različitim lokacijama diljem Italije. Sekvencionirani su geni cob, cox1 i 16S-rDNA, a dobivene su sekvencije uspoređene s odgovarajućim sekvencijama u online bazama podataka BOLD i GenBank. Ova metoda omogućuje brzo i točno utvrđivanje genetičkog porijekla rakova, mekušaca i riba, što se može ubuduće primijeniti u svrhu zaštite ugroženih vrsta, te sprečavanje njihove ilegalne prodaje. Također se ovom metodom može utvrditi istinitost podataka na deklaracijama prehrambenih proizvoda od riba, rakova i mekušaca.

Keywords

„DNA barkodiranje“; genetska sljedivost; baza podataka BOLD; rakovi, ribe i mekušci; deklaracije na proizvodima

Hrčak ID:

94367

URI

https://hrcak.srce.hr/94367

Publication date:

27.12.2012.

Article data in other languages: english

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