Izvorni znanstveni članak
DNA Barcoding as a Reliable Method for the Authentication of Commercial Seafood Products
Silvia Nicolè
; Laboratory of Genetics and Genomics, Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
Enrico Negrisolo
; Department of Comparative Biomedicine and Food Science, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
Giulia Eccher
; Laboratory of Genetics and Genomics, Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
Roberto Mantovani
; Laboratory of Genetics and Genomics, Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
Tomaso Patarnello
; Department of Comparative Biomedicine and Food Science, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
David L. Erickson
; Department of Botany and Laboratories of Analytical Biology, National Museum of Natural History, Smithsonian Institution, P.O. Box 37012, Washington, DC 20013-7012, USA
W. John Kress
; Department of Botany and Laboratories of Analytical Biology, National Museum of Natural History, Smithsonian Institution, P.O. Box 37012, Washington, DC 20013-7012, USA
Gianni Barcaccia
orcid.org/0000-0001-7478-5048
; Laboratory of Genetics and Genomics, Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Campus of Agripolis, Viale dell’Università 16, IT-35020 Legnaro, Italy
Sažetak
Animal DNA barcoding allows researchers to identify different species by analyzing a short nucleotide sequence, typically the mitochondrial gene cox1. In this paper, we use DNA barcoding to genetically identify seafood samples that were purchased from various locations throughout Italy. We adopted a multi-locus approach to analyze the cob, 16S-rDNA and cox1 genes, and compared our sequences to reference sequences in the BOLD and GenBank online databases. Our method is a rapid and robust technique that can be used to genetically identify crustaceans, mollusks and fishes. This approach could be applied in the future for conservation, particularly for monitoring illegal trade of protected and endangered species. Additionally, this method could be used for authentication in order to
detect mislabeling of commercially processed seafood.
Ključne riječi
DNA barcoding; genetic traceability; BOLD; seafood; mislabeling
Hrčak ID:
94367
URI
Datum izdavanja:
27.12.2012.
Posjeta: 3.449 *