Izvorni znanstveni članak
https://doi.org/10.15255/KUI.2025.003
Green Synthesis of Silver Nanoparticles Using Green Tea: Characterisation and Antibacterial Properties
Damir Barbir
orcid.org/0000-0002-5298-9004
; Sveučilište u Splitu, Kemijsko-tehnološki fakultet, R. Boškovića 35, 21 000 Split
*
Pero Dabić
orcid.org/0000-0002-6803-4624
; Sveučilište u Splitu, Kemijsko-tehnološki fakultet, R. Boškovića 35, 21 000 Split
Ivana Weber
; Sveučilište u Splitu, Prirodoslovno-matematički fakultet, R. Boškovića 33, 21 000 Split
Anita Rakić
; Nastavni zavod za javno zdravstvo Splitsko-dalmatinske županije, Vukovarska 46, 21 000 Split
* Dopisni autor.
Sažetak
In this study, silver nanoparticles were synthesised through an eco-friendly process without the use of organic or toxic solvents. The synthesis was carried out with green tea serving as both the reducing and stabilising agent, using 1 mM silver nitrate solution and distilled water as solvent. Catechins present in green tea were involved in silver ion reduction. Characterisation of the colloidal silver was conducted using UV-Vis spectrophotometry, FTIR spectroscopy, dynamic light scattering (DLS) and scanning electron microscopy (SEM). UV-Vis spectrophotometer confirmed the presence of silver nanoparticles through the observation of surface plasmon resonance, while DLS and SEM analyses were employed to assess nanoparticle size, shape, and sample uniformity. DLS analysis revealed particle distribution in colloidal silver with nanoparticle sizes ranging between 32 and 95 nm.
FTIR analysis confirmed the presence of polyphenols, particularly catechins, in green tea, emphasising the role of OH groups in the reduction of silver ions and nanoparticle synthesis. SEM analysis showed that the silver nanoparticles were spherical, with an average size of 62 nm. The antibacterial efficacy of the silver nanoparticles was tested against Escherichia coli NCTC 13216 and Staphylococcus aureus ATCC 25923. The results demonstrated an antimicrobial effect of green tea-synthesised silver nanoparticles against both bacteria at specific concentrations.
Ključne riječi
green synthesis; silver nanoparticles; green tea; Staphylococcus aureus; Escherichia coli
Hrčak ID:
334370
URI
Datum izdavanja:
13.9.2025.
Posjeta: 368 *