Short communication, Note

Identification of Wine Yeasts by PCR-RFLP without Previous Isolation on Plate

Juan Carlos Espinosa ; Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias Químicas, Universidad de Castilla-La Mancha, Avda. Camilo José Cela s/n., E-13071 Ciudad Real, Spain
Monica Fernandez-Gonzalez ; Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias Químicas, Universidad de Castilla-La Mancha, Avda. Camilo José Cela s/n., E-13071 Ciudad Real, Spain
Juan Ubeda ; Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias Químicas, Universidad de Castilla-La Mancha, Avda. Camilo José Cela s/n., E-13071 Ciudad Real, Spain
Ana Briones ; Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias Químicas, Universidad de Castilla-La Mancha, Avda. Camilo José Cela s/n., E-13071 Ciudad Real, Spain

Abstract

The population of wine yeasts during spontaneous must fermentation was characterized by direct 5.8S-ITS rDNA region amplification without previous plate isolation or enrichment. RFLP analysis was applied to each of the amplification products detected, and the corresponding yeast identifications were made. The method provides a fast and direct way of determining yeast population present during wine fermentation.

Keywords

wine yeasts; identification; PCR-RFLP

Hrčak ID:

178448

URI

https://hrcak.srce.hr/178448

Publication date:

18.6.2002.

Article data in other languages: croatian

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