Original scientific paper

Antimikrobna rezistencija koagulaza-negativnih stafilokoka iz tradicionalnih fermentiranih kobasica

Nevijo Zdolec orcid.org/0000-0001-7413-8115 ; University of Zagreb, Faculty of Veterinary Medicine, Department of Food Hygiene, Technology and Safety, Heinzelova 55, HR-10000 Zagreb, Croatia
Ivana Račić ; Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Savska cesta 143, HR-10000 Zagreb, Croatia
Anja Vujnović ; Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Savska cesta 143, HR-10000 Zagreb, Croatia
Maja Zdelar-Tuk ; Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Savska cesta 143, HR-10000 Zagreb, Croatia
Krešimir Matanović ; University of Zagreb, Faculty of Veterinary Medicine, Department of Microbiology and Infectious Diseases with Clinic, Heinzelova 55, HR-10000 Zagreb, Croatia
Ivana Filipović ; University of Zagreb, Faculty of Veterinary Medicine, Department of Food Hygiene, Technology and Safety, Heinzelova 55, HR-10000 Zagreb, Croatia
Vesna Dobranić ; University of Zagreb, Faculty of Veterinary Medicine, Department of Food Hygiene, Technology and Safety, Heinzelova 55, HR-10000 Zagreb, Croatia
Željko Cvetnić ; Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Savska cesta 143, HR-10000 Zagreb, Croatia
Silvio Špičić ; Croatian Veterinary Institute, Department for Bacteriology and Parasitology, Laboratory for Bacterial Zoonoses and Molecular Diagnostics of Bacterial Diseases, Savska cesta 143, HR-10000 Zagreb, Croatia

Abstract

U radu je primjenom klasičnih i molekularnih metoda istražena osjetljivost koagulaza-negativnih stafilokoka iz tradicionalnih fermentiranih kobasica na antimikrobne spojeve. Pomoću disk-difuzijskog i epsilometar testa ispitana je osjetljivost izolata na vankomicin, ampicilin, eritromicin, tetraciklin, gentamicin i oksacilin. Metodom je PCR u izolatima određena prisutnost gena za rezistenciju, i to mecA, ermB, tetK i tetM. Utvrđene su sljedeće vrste koagulaza-negativnih stafilokoka: Staphylococcus epidermidis (69 %), S. capitis (5 %) i S. warneri (2.5 %). Ukupno je 44,4 % izolata S. epidermidis pokazalo fenotipsku rezistenciju na tetraciklin i eritromicin. Prisutnost gena tetK ili tetM potvrđena je u 8 izolata S. epidermidis. Iako S. epidermidis nije uobičajeni uzročnik trovanja hranom, njegova je prisutnost u hrani važna za javno zdravstvo zbog mogućeg širenja antimikrobne rezistencije. Dobiveni rezultati pokazuju da spontanom fermentacijom mesa mogu nastati proizvodi opasni za zdravlje potrošača.

Keywords

fermentirane kobasice; Staphylococcus epidermidis; geni za rezistenciju

Hrčak ID:

104817

URI

https://hrcak.srce.hr/104817

Publication date:

28.6.2013.

Article data in other languages: english

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