Izvorni znanstveni članak
The Importance of Serological Tests Implementation in Disseminated Candidiasis Diagnose
Merima Gegic
; Tuzla University Clinic Centre, Clinic for Laboratory Diagnosis, Department of Microbiology, Tuzla, Bosnia and Herzegovina
Fatima Numanovic
; Tuzla University Clinic Centre, Clinic for Laboratory Diagnosis, Department of Microbiology, Tuzla, Bosnia and Herzegovina
Zineta Delibegovic
; Tuzla University Clinic Centre, Clinic for Laboratory Diagnosis, Department of Microbiology, Tuzla, Bosnia and Herzegovina
Nijaz Tihic
; Tuzla University Clinic Centre, Clinic for Laboratory Diagnosis, Department of Microbiology, Tuzla, Bosnia and Herzegovina
Mahmut Nurkic
; Tuzla University Clinic Centre, Clinic for Laboratory Diagnosis, Department of Microbiology, Tuzla, Bosnia and Herzegovina
Mirsada Hukic
; University of Sarajevo Clinics Centre, Institute for Clinical Microbiology, Sarajevo, Bosnia and Herzegovina
Sažetak
Candidiasis is defined as an infection or disease caused by a fungus of the genus Candida. Rate of disseminated candidiasis increases with the growth of the number of immunocompromised patients. In the the last few decades the incidence of disseminated candidiasis is in growth as well as the mortality rate. The aim of this survey is to show the importance of serological tests implemenation in disseminated candidiasis diagnose. This is a prospective study involving 60 patients with malign diseases with and without clinical signs of disseminated candidiasis and 30 healthy people who represent the control group. Apart from hemoculture, detection of circulating mannan antigen and adequate antibodies of Candida species applying comercial ELISA test was determined in each patient. This survey deals with relevant factors
causing disseminated candidiasis. This survey showed that the group of patients with clinical signs of disseminated canididiasis had more patients with positive hemoculture to Candida species, then the group of patients without clinical signs of disseminated candidiasis. The number of patients being examined and positive to antigens and antibodies was higher (p<0.01) in the group of patients with clinical signs of disseminated candidiasis (7/30; 23.3%), then in the group of patients without clinical signs of disseminated candidiasis (0/30; 0%). Average value of titra antigen was statistically higher (p<0.001) in patients with Candida spp. positive hemocultures rather then in patients with Candida spp. negative hemocultures. In the group of patients with clinical signs of disseminated candidiasis 6/30 (20%) of patients had Candida spp.positive hemocultures while in the group of patients without clinical signs of disseminated candidiasis 1/30 (3.3%) of patients had Candida spp. positive hemocultures, which was considerably higher (p<0.05). Correlation of results of hemoculture and mannan antigens and antibodies in patients with disseminated candidiasis were statistically significant, while correlation of results of hemoculture and antibodies was insignificant. Because of low sensitivity of hemoculture and time needed for isolation of Candida spp., introducing serological tests in regular procedures would speed disseminated candidiasis diagnose.
Ključne riječi
Candida; mannan antigen; disseminated candidiasis
Hrčak ID:
99549
URI
Datum izdavanja:
3.4.2013.
Posjeta: 937 *