Veterinarski arhiv, Vol. 84 No. 3, 2014.
Izvorni znanstveni članak
Identification, localization and quantification of porcine intestinal immune cell subsets during the first seven weeks of postnatal ontogeny.
Damir Žubčić
; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ana Kovšca Janjatović
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Dubravko Kezić
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Daniel Špoljarić
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Hrvoje Valpotić
; Department of Animal Nutrition and Dietetics, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Gordana Lacković
; Department of Zoology, Division of Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Deny Anđelinović
; KBC Split, Croatia
Gordan Mršić
; Forensic Sciences Center “Ivan Vučetić”, General Police Directorate, Ministry of the Interior of the Republic of Croatia, Zagreb, Croatia
Maja Popović
; Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
By using immunohistology and digital image analysis for histomorphometry, we investigated the influence of porcine postnatal development on distribution and quantitative patterns of naïve/memory lymphoid cell subsets (CD45RA+, CD45RC+, respectively), helper/cytotoxic T cell subsets (CD4+, CD8+, respectively) as well as of IgA+ plasma cells in the gut-associated lymphoid tissues (GALT) of pigs reared under intensive conditions during the first 7 weeks of life. Current research on the postnatal ontogenesis/maturation of porcine small intestinal mucosal immune system have shown that newborn pigs are immunologically rather immature and, thus, totally dependent on passively acquired immunity. Their jejunal mucosa almost lacks immune cells, with the exception of a low number of CD45RA+ lymphoid cells (1.25×10-5) at Day 0 post partum. A very few CD45RC+ lymphoid cells (8.79×10-6 ) appeared at Day 7, followed by a similar number of IgA+ plasma cells (7.65×10-6) which were observed at Day 14. Such first signs of the postnatal development of both innate and adaptive immunity by identification/localization of tested cell subsets residing porcine GALT, i e. jejunal mucosal sites are proceeded by a lag period of three weeks until the appearance of low quantities of CD4+ (3.62×10-5) and CD8+ (3.13×10-5) T cells at Day 35 after birth. After that phase of early postnatal development of the crucial cell subsets participating in the antigen-specific immunity on the gut mucosal surfaces of young pigs, further progress was visible through their different distribution patterns within the jejunal mucosa compartments, and a gradual increase in their numbers, particularly between Day 35 and Day 49 of age. A significant increase was recorded within that period in the numbers of naïve, memory and plasma cells, whereas the number of helper and cytotoxic T cells only slightly increased between Day 42 and Day 49 of life.
Ključne riječi
postnatal development; immune cells; gut mucosa; pigs
Hrčak ID:
122088
URI
Datum izdavanja:
28.5.2014.
Posjeta: 1.172 *