Veterinarski arhiv, Vol. 85 No. 6, 2015.
Izvorni znanstveni članak
Molecular detection and characterization of velogenic Newcastle disease virus in common starlings in Macedonia.
Aleksandar Dodovski
; Department of Avian Diseases, Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius”, Skopje, Macedonia
Kiril Krstevski
; Department of Infectious Diseases, Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius” Skopje, Macedonia
Igor Dzadzovski
; Department of Farm Animal Health, Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius” Skopje, Macedonia
Ivancho Naletoski
; Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria
Sažetak
The objective of this study was to assess virulence using molecular methods and to phylogenetically characterize Newcastle disease virus (NDV) detected in common starlings (Sturnus vulgaris) in Macedonia. Nucleotide sequencing of the cleavage site of the F gene revealed an amino acid pattern specific for virulent strains of NDV with phenylalanine at position 117 and multiple basic amino acids between positions 112 and 116, i.e. 112RRQKR*FIG119. The 374 bp region of the fusion (F) gene used for phylogenetic analyses revealed that the detected strain belongs to subgenotype VIId of the class II NDV, and it is similar to virulent viruses detected in back-yard poultry in Macedonia in 2005 and 2006, as well as to viruses from poultry and wild birds detected in Serbia and Bulgaria in 2006 and 2007. Epidemiological data suggest that the common starlings were probably infected as a result of an ongoing epizootic in domestic poultry and they did not have a role in the primary introduction and spread of the virus to domestic poultry. This study represents the first report of the detection and molecular and phylogenetic characterization of virulent NDV from wild birds in Macedonia.
Ključne riječi
common starling; Newcastle disease virus; molecular characterization
Hrčak ID:
149856
URI
Datum izdavanja:
9.12.2015.
Posjeta: 1.575 *