Izvorni znanstveni članak
Population Genetic Analysis of Haplotypes Based on 17 Short Tandem Repeat Loci on Y Chromosome in Population Sample from Eastern Croatia
Branka Gršković
; Forensic Science Centre “Ivan Vučetić”, General Police Directorate Ministry of Interior, Zagreb, Croatia
Gordan Mršić
; Forensic Science Centre “Ivan Vučetić”, General Police Directorate Ministry of Interior, Zagreb, Croatia. University Center for Forensic Sciences, University of Split, Split,Croatia
Andro Vrdoljak
; Forensic Science Centre “Ivan Vučetić”, General Police Directorate Ministry of Interior, Zagreb, Croatia. University Center for Forensic Sciences, University of Split, Split,Croatia
Siniša Merkaš
; Forensic Science Centre “Ivan Vučetić”, General Police Directorate Ministry of Interior, Zagreb, Croatia
Šimun Anđelinović
; University Center for Forensic Sciences, University of Split, Split,Croatia. Department of Pathology and Forensic Medicine, Split University Hospital and School of Medicine, Split, Croatia
Sažetak
Aim To investigate the population genetics of 17 short tandem
repeat (STR) loci on the Y chromosome in the population
of eastern Croatia.
Methods We carried out a statistical analysis of the data
from previously performed genetic analysis collected during
routine forensic work by the Forensic Science Centre
“Ivan Vučetić”. A total of 220 unrelated healthy men from
eastern Croatia were selected for the purpose of this study.
Genomic DNA was extracted by Chelex from FTA® cards.
Y-chromosomal STRs were determined using the AmpFISTR
Yfiler PCR amplification kit. The haplotype frequencies
were determined by direct counting and analyzed using
Arlequin 3.1 and analysis of molecular variance calculated
with the Y-chromosome haplotype reference database online
analysis tool.
Results A total of 207 haplotypes were recorded, 197 of
which were unique (90%). Haplotype diversity was 0.9993,
with the most frequent haplotype found in 4 of 220 men
(1.8%). Average locus diversity was 0.600, and it ranged
from 0.256 for DYS392 to 0.780 for DYS458. Our results were
compared with the pattern of Y-chromosome variability in
publicly available population samples based on a minimal
European haplotype set of 9 STRs and the greatest resemblance
was found with samples from the Croatian capital
of Zagreb, from Bosnia and Herzegovina, and from Serbia.
Conclusion This is the first description of Y chromosome
haplotyping of the population of eastern Croatia, which
may serve as a basis for genetic epidemiology and forensic
studies. Further studies are needed for characterization of
the genetic structure of the Y-chromosome in the modern
Croatian population
Ključne riječi
Eastern Croatia; haplotypes; population genetics; short tandem repeat; Y chromosome
Hrčak ID:
55655
URI
Datum izdavanja:
15.6.2010.
Posjeta: 1.668 *