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Periodicum biologorum, Vol.112 No.2 Lipanj 2010.

Izvorni znanstveni članak

Genetic characterization of broodstock brown trout from Bled fish-farm, Slovenia

SAŠA MARIĆ ; Institute of Zoology, Faculty of Biology, University of Belgrade, Studentski Trg 16, 11000 Belgrade, Serbia
PREDRAG SIMONOVIĆ ; Institute of Zoology, Faculty of Biology, University of Belgrade, Studentski Trg 16, 11000 Belgrade, Serbia
ANDREJ RAZPET ; Department of Animal Science Biotechnical Faculty, University of Ljubljana, Groblje 3, 1230 Domžale, Slovenia

Puni tekst: pdf (120 KB), Engleski, Str. 145 - 148 , preuzimanja: 277 *

Sažetak
Background and Purpose: Due to environmental and economic concerns, Bled fish-farm is interested in establishing broodstocks of native brown trout (Salmo trutta L.). Progeny would be reared and released into rivers managed by the Fishing Club Bled. In this study was performed genetic characterization of broodstock from Bled fish-farm in order to assess hybridization of native brown trout of Danubian phylogeographic lineage with trout of the allochtonous Atlantic lineage.

Material and Methods: DNA was isolated from fin clips of 20 males
and 20 females from broodstock. PCR-RFLP technique was used for distinguishing between Atlantic and Danubian lineages on the basis of control region of the mitochondrialDNA(CRmtDNA) and lactate dehydrogenase gene (LDH).

Results: Results show a high percentage of allochthonous genetic markers especially among females and confirm hybridization between native and introduced brown trout.

Conclusions: Because of low percentage of native trout markers, Bled
fish-farm decided to continue with collecting and genotyping fish to establish a broodstock with higher percentage of genetic markers characteristic for native trout of Danubian lineage.

Ključne riječi
Salmo trutta; fish farm; broodstock; genetic characterization; Slovenia

Posjeta: 404 *