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https://doi.org/10.15255/CABEQ.2014.2250

Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains

C. Haas ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)
V. Steinwandter ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)
E. Diaz De Apodaca ; TECNALIA, Parque Tecnológico de Álava, 01510 Miñano (Spain)
B. Maestro Madurga ; TECNALIA, Parque Tecnológico de Álava, 01510 Miñano (Spain)
M. Smerilli ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)
T. Dietrich ; TECNALIA, Parque Tecnológico de Álava, 01510 Miñano (Spain)
M. Neureiter orcid id orcid.org/0000-0002-0202-6832 ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)


Puni tekst: engleski pdf 1.596 Kb

str. 99-112

preuzimanja: 913

citiraj


Sažetak

Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L–1 sugars and 0.7 g L–1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L–1 with a PHB content of 66 % in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L–1 dry biomass containing 46 % PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L–1 of biomass containing 78 % PHB after only 3 days were obtained. These results show that even within the same species, the productivities on natural substrates are very different. The produced polymers were extracted using chloroform, and several thermo-physical parameters are in good accordance with published data. Overall, our holistic approach and the encouraging results prove that chicory roots are a viable fermentation substrate for PHB-production.

Ključne riječi

Cichorium intybus, fructose, inulin, by-product, food residue, pre-treatment, hydrolysate, batch fermentation, Cupriavidus necator, P3HB, polymer characterisation

Hrčak ID:

141905

URI

https://hrcak.srce.hr/141905

Posjeta: 1.660 *