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Original scientific paper
https://doi.org/10.15255/CABEQ.2014.2250

Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains

C. Haas ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)
V. Steinwandter ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)
E. Diaz De Apodaca ; TECNALIA, Parque Tecnológico de Álava, 01510 Miñano (Spain)
B. Maestro Madurga ; TECNALIA, Parque Tecnológico de Álava, 01510 Miñano (Spain)
M. Smerilli ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)
T. Dietrich ; TECNALIA, Parque Tecnológico de Álava, 01510 Miñano (Spain)
M. Neureiter   ORCID icon orcid.org/0000-0002-0202-6832 ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)

Fulltext: english, pdf (2 MB) pages 99-112 downloads: 554* cite
APA 6th Edition
Haas, C., Steinwandter, V., Diaz De Apodaca, E., Maestro Madurga, B., Smerilli, M., Dietrich, T. & Neureiter, M. (2015). Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains. Chemical and Biochemical Engineering Quarterly, 29 (2), 99-112. https://doi.org/10.15255/CABEQ.2014.2250
MLA 8th Edition
Haas, C., et al. "Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains." Chemical and Biochemical Engineering Quarterly, vol. 29, no. 2, 2015, pp. 99-112. https://doi.org/10.15255/CABEQ.2014.2250. Accessed 1 Jun. 2020.
Chicago 17th Edition
Haas, C., V. Steinwandter, E. Diaz De Apodaca, B. Maestro Madurga, M. Smerilli, T. Dietrich and M. Neureiter. "Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains." Chemical and Biochemical Engineering Quarterly 29, no. 2 (2015): 99-112. https://doi.org/10.15255/CABEQ.2014.2250
Harvard
Haas, C., et al. (2015). 'Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains', Chemical and Biochemical Engineering Quarterly, 29(2), pp. 99-112. https://doi.org/10.15255/CABEQ.2014.2250
Vancouver
Haas C, Steinwandter V, Diaz De Apodaca E, Maestro Madurga B, Smerilli M, Dietrich T, et al. Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains. Chemical and Biochemical Engineering Quarterly [Internet]. 2015 [cited 2020 June 01];29(2):99-112. https://doi.org/10.15255/CABEQ.2014.2250
IEEE
C. Haas, et al., "Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains", Chemical and Biochemical Engineering Quarterly, vol.29, no. 2, pp. 99-112, 2015. [Online]. https://doi.org/10.15255/CABEQ.2014.2250

Abstracts
Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L–1 sugars and 0.7 g L–1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L–1 with a PHB content of 66 % in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L–1 dry biomass containing 46 % PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L–1 of biomass containing 78 % PHB after only 3 days were obtained. These results show that even within the same species, the productivities on natural substrates are very different. The produced polymers were extracted using chloroform, and several thermo-physical parameters are in good accordance with published data. Overall, our holistic approach and the encouraging results prove that chicory roots are a viable fermentation substrate for PHB-production.

Keywords
Cichorium intybus; fructose; inulin; by-product; food residue; pre-treatment; hydrolysate; batch fermentation; Cupriavidus necator; P3HB; polymer characterisation

Hrčak ID: 141905

URI
https://hrcak.srce.hr/141905

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