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Chemical and Biochemical Engineering Quarterly, Vol. 29 No. 2, 2015

Original scientific paper
https://doi.org/10.15255/CABEQ.2014.2250

Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains

C. Haas ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)
V. Steinwandter ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)
E. Diaz De Apodaca ; TECNALIA, Parque Tecnológico de Álava, 01510 Miñano (Spain)
B. Maestro Madurga ; TECNALIA, Parque Tecnológico de Álava, 01510 Miñano (Spain)
M. Smerilli ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)
T. Dietrich ; TECNALIA, Parque Tecnológico de Álava, 01510 Miñano (Spain)
M. Neureiter   ORCID icon orcid.org/0000-0002-0202-6832 ; University of Natural Resources and Life Sciences, Vienna, IFA Tulln, Institute for Environmental Biotechnology, 3430 Tulln (Austria)

Fulltext: english, pdf (2 MB) pages 99-112 downloads: 431* cite
APA 6th Edition
Haas, C., Steinwandter, V., Diaz De Apodaca, E., Maestro Madurga, B., Smerilli, M., Dietrich, T. & Neureiter, M. (2015). Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains. Chemical and Biochemical Engineering Quarterly, 29 (2), 99-112. https://doi.org/10.15255/CABEQ.2014.2250
MLA 8th Edition
Haas, C., et al. "Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains." Chemical and Biochemical Engineering Quarterly, vol. 29, no. 2, 2015, pp. 99-112. https://doi.org/10.15255/CABEQ.2014.2250. Accessed 25 May 2019.
Chicago 17th Edition
Haas, C., V. Steinwandter, E. Diaz De Apodaca, B. Maestro Madurga, M. Smerilli, T. Dietrich and M. Neureiter. "Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains." Chemical and Biochemical Engineering Quarterly 29, no. 2 (2015): 99-112. https://doi.org/10.15255/CABEQ.2014.2250
Harvard
Haas, C., et al. (2015). 'Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains', Chemical and Biochemical Engineering Quarterly, 29(2), pp. 99-112. doi: https://doi.org/10.15255/CABEQ.2014.2250
Vancouver
Haas C, Steinwandter V, Diaz De Apodaca E, Maestro Madurga B, Smerilli M, Dietrich T, et al. Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains. Chemical and Biochemical Engineering Quarterly [Internet]. 2015 [cited 2019 May 25];29(2):99-112. doi: https://doi.org/10.15255/CABEQ.2014.2250
IEEE
C. Haas, et al., "Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains", Chemical and Biochemical Engineering Quarterly, vol.29, no. 2, pp. 99-112, 2015. [Online]. doi: https://doi.org/10.15255/CABEQ.2014.2250

Abstracts
Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L–1 sugars and 0.7 g L–1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L–1 with a PHB content of 66 % in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L–1 dry biomass containing 46 % PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L–1 of biomass containing 78 % PHB after only 3 days were obtained. These results show that even within the same species, the productivities on natural substrates are very different. The produced polymers were extracted using chloroform, and several thermo-physical parameters are in good accordance with published data. Overall, our holistic approach and the encouraging results prove that chicory roots are a viable fermentation substrate for PHB-production.

Keywords
Cichorium intybus; fructose; inulin; by-product; food residue; pre-treatment; hydrolysate; batch fermentation; Cupriavidus necator; P3HB; polymer characterisation

Hrčak ID: 141905

URI
https://hrcak.srce.hr/141905

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