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https://doi.org/10.2478/v10007-010-0035-6

A novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis: Application in the analysis of certain antihypertensive drugs

IBRAHIM A. DARWISH ; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
ASHRAF M. MAHMOUD ; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
ABDUL-RAHMAN A. AL-MAJED ; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia

Fulltext: english, pdf (73 KB) pages 493-501 downloads: 271* cite
APA 6th Edition
DARWISH, I.A., MAHMOUD, A.M. & AL-MAJED, A.A. (2010). A novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis: Application in the analysis of certain antihypertensive drugs. Acta Pharmaceutica, 60 (4), 493-501. https://doi.org/10.2478/v10007-010-0035-6
MLA 8th Edition
DARWISH, IBRAHIM A., et al. "A novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis: Application in the analysis of certain antihypertensive drugs." Acta Pharmaceutica, vol. 60, no. 4, 2010, pp. 493-501. https://doi.org/10.2478/v10007-010-0035-6. Accessed 17 Oct. 2019.
Chicago 17th Edition
DARWISH, IBRAHIM A., ASHRAF M. MAHMOUD and ABDUL-RAHMAN A. AL-MAJED. "A novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis: Application in the analysis of certain antihypertensive drugs." Acta Pharmaceutica 60, no. 4 (2010): 493-501. https://doi.org/10.2478/v10007-010-0035-6
Harvard
DARWISH, I.A., MAHMOUD, A.M., and AL-MAJED, A.A. (2010). 'A novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis: Application in the analysis of certain antihypertensive drugs', Acta Pharmaceutica, 60(4), pp. 493-501. https://doi.org/10.2478/v10007-010-0035-6
Vancouver
DARWISH IA, MAHMOUD AM, AL-MAJED AA. A novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis: Application in the analysis of certain antihypertensive drugs. Acta Pharm. [Internet]. 2010 [cited 2019 October 17];60(4):493-501. https://doi.org/10.2478/v10007-010-0035-6
IEEE
I.A. DARWISH, A.M. MAHMOUD and A.A. AL-MAJED, "A novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis: Application in the analysis of certain antihypertensive drugs", Acta Pharmaceutica, vol.60, no. 4, pp. 493-501, 2010. [Online]. https://doi.org/10.2478/v10007-010-0035-6

Abstracts
The present study describes the development of a novel analytical approach that can reduce by 50-fold the consumption of organic solvents in the charge transfer (CT)-based spectrophotometric analysis. The proposed approach employed 96-microwell assay plates for carrying out the reaction. The CT reaction between the electron-donating analyte and electron-accepting reagent was performed in microwells (200-µL of organic solvent) and the color signals were measured with a microwell-plate reader. Optimum conditions for the proposed approach were established for two antihypertensive drugs, namely ramipril (RML) and lisinopril (LSL) as model compounds for the electron-donating analytes, and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) as a -electron acceptor. Under the optimum conditions, Beer’s law was obeyed in the concentration range of 6–100 and 6–60 g mL1 for RML and LSL, respectively. The limits of detection were 0.97 and 1.10 g mL1 for RML and LSL, respectively. The precision of the methods was satisfactory; the values of relative standard deviations did not exceed 1.1 %. The proposed approach was successfully applied to the analysis of pharmaceutical dosage forms with good accuracy and precision. The results were comparable with those of the reported methods. The approach described herein is of great practical value in pharmaceutical analysis because it reduces the exposure of analysts to the toxic effects of organic solvents, lowers the analysis cost by 50-fold, and it has a high throughput property. Although the approach was validated for RML and LSL, the same methodology could be used for any electron-donating analyte for which a CT-reaction can be performed.

Keywords
charge-transfer reaction; organic solvent; spectrophotometry; ramipril; lisinopril

Hrčak ID: 59303

URI
https://hrcak.srce.hr/59303

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