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Original scientific paper
https://doi.org/10.2478/10004-1254-63-2012-2218

Comparison of Buffers for Extraction of Mite Allergen Der p 1 from Dust

Ljerka Prester ; Institute for Medical Research and Occupational Health Zagreb, Croatia
Jelena Kovačić ; Institute for Medical Research and Occupational Health Zagreb, Croatia
Jelena Macan ; Institute for Medical Research and Occupational Health Zagreb, Croatia

Fulltext: english, pdf (100 KB) pages 293-299 downloads: 1.146* cite
APA 6th Edition
Prester, Lj., Kovačić, J. & Macan, J. (2012). Comparison of Buffers for Extraction of Mite Allergen Der p 1 from Dust. Arhiv za higijenu rada i toksikologiju, 63 (3), 293-299. https://doi.org/10.2478/10004-1254-63-2012-2218
MLA 8th Edition
Prester, Ljerka, et al. "Comparison of Buffers for Extraction of Mite Allergen Der p 1 from Dust." Arhiv za higijenu rada i toksikologiju, vol. 63, no. 3, 2012, pp. 293-299. https://doi.org/10.2478/10004-1254-63-2012-2218. Accessed 26 Oct. 2020.
Chicago 17th Edition
Prester, Ljerka, Jelena Kovačić and Jelena Macan. "Comparison of Buffers for Extraction of Mite Allergen Der p 1 from Dust." Arhiv za higijenu rada i toksikologiju 63, no. 3 (2012): 293-299. https://doi.org/10.2478/10004-1254-63-2012-2218
Harvard
Prester, Lj., Kovačić, J., and Macan, J. (2012). 'Comparison of Buffers for Extraction of Mite Allergen Der p 1 from Dust', Arhiv za higijenu rada i toksikologiju, 63(3), pp. 293-299. https://doi.org/10.2478/10004-1254-63-2012-2218
Vancouver
Prester Lj, Kovačić J, Macan J. Comparison of Buffers for Extraction of Mite Allergen Der p 1 from Dust. Arh Hig Rada Toksikol. [Internet]. 2012 [cited 2020 October 26];63(3):293-299. https://doi.org/10.2478/10004-1254-63-2012-2218
IEEE
Lj. Prester, J. Kovačić and J. Macan, "Comparison of Buffers for Extraction of Mite Allergen Der p 1 from Dust", Arhiv za higijenu rada i toksikologiju, vol.63, no. 3, pp. 293-299, 2012. [Online]. https://doi.org/10.2478/10004-1254-63-2012-2218

Abstracts
Der p 1 is the main allergen of house dust mite Dermatophagoides pteronyssinus, which has routinely been detected in residential dust. However, the procedure for extracting Der p 1 from reservoir dust has not been well defined. The aim of this study was to compare Der p 1 mass fractions in dust extracts prepared using the following extraction buffers: phosphate (pH 7.4), borate (pH 8.0), and ammonium bicarbonate (pH 8.0), all with 0.05 % Tween 20. Twenty-eight dust samples were divided into three aliquots and each portion was extracted with one of the three buffers at room temperature. Der p 1 mass fractions were measured in a total of 84 dust extracts using the enzyme immunoassay (range: 0.1 μg g-1 to 7.53 μg g-1). Statistical methods including intraclass correlation showed a high agreement between Der p 1 mass fractions irrespective of the extracting medium. Our results suggest that all three buffers are suitable for the extraction of mite allergens and routine Der p 1 analysis in dust.

Keywords
Dermatophagoides pteronyssinus; ELISA; extraction buffers; indoor allergens; intraclass correlation; settled dust

Hrčak ID: 86887

URI
https://hrcak.srce.hr/86887

[croatian]

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