Veterinarski arhiv, Vol. 81 No. 3, 2011.
Izvorni znanstveni članak
Polymorphism in 3’UTR region of Slc11a1 gene in Indian breeds of cattle.
Rakesh Ranjan
; Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidhyalya, Jabalpur, Madhya Pradesh, India
Chandrakant D. Bhong
; OME Research Facility, Department of Animal Biotechnology, College of Veterinary Science and A. H., Anand, Anand Agricultural University, Anand Gujarat, India
Khagol N. Chavan
; Department of Animal Genetics and Breeding, College of Veterinary Science and A. H., S. K. Nagar, SKDAU, S. K. Nagar, Gujarat, India
S. N. S. Parmar
; Department of Animal Breeding and Genetics, College of Veterinary Science and A. H., Jabalpur, JNKVV, Jabalpur, Madhya Pradesh, India
Chaitanya G. Joshi
; OME Research Facility, Department of Animal Biotechnology, College of Veterinary Science and A. H., Anand, Anand Agricultural University, Anand Gujarat, India
Sažetak
The solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (Slc11a1) also called natural resistance-associated macrophage protein 1 gene (Nramp1) is a member of the large family of metal ion-transport proteins. It encodes a divalent cation (Fe+ & Mn+) transporter that localizes in the phagolysosome membrane in macrophages. Slc11a1 gene plays a critical role in innate immunity favoring bacterial killing by macrophages in addition to its influence on adaptative immunity. Polymorphism at the 3’ untranslated region (3’UTR) of Slc11a1 gene is associated with natural resistance against brucellosis in cattle. Such polymorphisms are associated with variations in the number of GT repeats. This study aimed to discover polymorphism in the 3’UTR region of the Slc11a1 gene in the Indian cattle breeds Nimari and Kenkatha. Polymerase Chain Reaction - Single Strand Conformation Polymorphism (PCR-SSCP) of 440 bp amplicon of Slc11a1 gene revealed three common SSCP patterns in these breeds, which was also confirmed by detecting point mutation in sequences of these patterns. The study will augment the information available and be useful in further studies to determine the role of the Slc11a1 gene in disease resistance and for the selection of brucellosis resistant animals.
Ključne riječi
cattle; Slc11a1 gene; 3’UTR; polymerase chain reaction - single strand conformation polymorphism
Hrčak ID:
69440
URI
Datum izdavanja:
20.6.2011.
Posjeta: 1.361 *