Veterinary Archives, Vol. 73 No. 1, 2003.
Original scientific paper
Kinetics of antibody response of calves immunized with Schistosoma mansoni glutathione-S-transferase.
Imadeldin Aradaib
; Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Khartoum North, Sudan
Rihab Omer
; Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Khartoum North, Sudan
Ali Majid
; National Council for Research, Ministry of Science and Technology
Abstract
Six calves were immunized with Schistosoma mansoni glutathione-S transferase (GST) antigen vaccine, expressed in E. coli (GST-coli) or yeast (GST-yeast). Six calves were injected with extracts of E. coli or yeast and kept as controls. Two experimentally infected calves were kept as source of infection and to provide positive antiserum to Schistosoma bovis. Kinetics of antibody response of the immunized calves was monitored by Western blots and enzyme-linked immunosorbent assay (ELISA). Using Western blots, the immunized calves produced specific IgG antibodies, which recognized S. mansoni GST (S.mGST) antigen, whereas the control calves did not. Using ELISA, antibodies to S.mGST antigen were detected in sera from S.mGSTcoli and GST-yeast vaccinated calves, but not in sera from control calves or S. bovis experimentally infected calves. In addition, the ELISA failed to detect S.mGST specific antibodies in sera from immunized calves or their controls when S. bovis adult worm extract was used as an antigen. The results of this study indicated that S.mGST antigen has no diagnostic potential for detection of bovine schistosomosis, caused by S. bovis infection, in susceptible ruminants.
Keywords
schistosome; cattle; immune response; glutathione-S-transferase
Hrčak ID:
71120
URI
Publication date:
21.2.2003.
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