Pregledni rad
New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks
Cai-xia Li
; Institute of Forensic Science, Ministry of Public Security, Beijing, China
Gui-qiang Wang
; Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing, China
Wan-shui Li
; Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing, China
Jiang-ping Huang
; Chinese Peoples Public Security University, Beijing, China
Annquan Ji
; Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing, China
Lan Hu
; Key Laboratory of Forensic Genetics, Ministry of Public Security, Beijing, China
Sažetak
Aim To isolate mucosal cells of the perpetrator in a sexual
assault case from a complex mixture of his mucosal cells
and the victim’s skin by micromanipulation prior to genomic
analysis.
Methods To capture and analyze mucosal cells we used
the micromanipulation with on-chip low volume polymerase
chain reaction (LV-PCR). Consensus DNA profiles
were generated from 5 replicate experiments.
Results and conclusions We validated the use of micromanipulation
with on-chip LV-PCR for genomic analysis of
complex biological mixtures in a fatal rape case. The perpetrator’s
mucosal cells were captured from nipple swabs of
the victim, and a single-source DNA profile was generated
from cell mixtures. These data suggest that micromanipulation
with on-chip LV-PCR is an effective forensic tool for
the analysis of specific cells from complex samples.
Ključne riječi
Hrčak ID:
71439
URI
Datum izdavanja:
15.6.2011.
Posjeta: 1.583 *