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Competitive Adsorption of Three Human Plasma Proteins onto Sulfhydryl-to-sulfonate Gradient Surfaces

Yong-Xue Ding ; Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA
Vladimir Hlady ; Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA

Puni tekst: engleski pdf 9.084 Kb

str. 193-202

preuzimanja: 901



Competitive adsorption of three human plasma proteins: albumin (HSA), fibrinogen (Fgn), and
immunoglobulin G (IgG) from their ternary solution mixtures onto a sulfhydryl-to-sulfonate gradient surface
was investigated using spatially-resolved total internal reflection fluorescence (TIRF) and autoradiography.
The concentration of each protein in the ternary solution mixture was kept at an equivalent of
1/100 of its physiological concentration in blood plasma. The three proteins displayed different adsorption
and desorption characteristics. Each protein adsorbed less to the sulfonate region than to the sulfhydryl region
of the gradient. The adsorption-desorption kinetics revealed large differences in the adsorption and
desorption rates of three proteins. By fitting the experimental data to a simple model of competitive protein
adsorption, the affinity of each protein to the surface at the gradient center position was ranked as:
Fgn > HSA >> IgG. Competitive exchange of adsorbed proteins was related to the magnitude of desorption
rate constants. Such competitive adsorption of the three major human plasma proteins illustrates the
complex dynamics of blood proteins – biomaterials interactions.(doi: 10.5562/cca1821)

Ključne riječi

surface gradient; fibrinogen; albumin; immunoglobulin G; competitive protein adsorption

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