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https://doi.org/10.5562/cca1767

Flow Cytometric Analysis of the Influence of Myosmine on the Cell Cycle

Borislava I. Boteva ; Institute of Molecular Biology, Bulgarian Academy of Sciences, ul. Acad Bonchev 21, Sofia 1113, Bulgaria
Rada M. Mateva ; Institute of Molecular Biology, Bulgarian Academy of Sciences, ul. Acad Bonchev 21, Sofia 1113, Bulgaria
Ivan A. Iliev ; Institute for Experimental Morphology, Pathology and Anthropology with Museum (IEMPAM), ul. Acad Bonchev 25, Sofia1113, Bulgaria
Galina A. Gorneva ; Institute of Molecular Biology, Bulgarian Academy of Sciences, ul. Acad Bonchev 21, Sofia 1113, Bulgaria


Puni tekst: engleski pdf 3.313 Kb

str. 355-359

preuzimanja: 983

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Sažetak

Myosmine (3-(1-pyrrolin-2-yl)pyridine) is an alkaloid found in tobacco as well as various staple
foods, fruits and vegetables. Myosmine has recently been suspected to be a tobacco-independent carcinogenic
source. Using cell flow cytometry, we have examined the influence of myosmine on the cell cycle
of murine erythroleukemia (MEL) cells in vitro and have compared this with its effects on murine bone
marrow cells in vivo. Myosmine at low concentrations inhibited cell proliferation dose dependently; while
at concentrations close to 300 μmol dm−3 it acted in a cytostatic fashion, that is, it increased the percentage
of cells in the S and G2/M phases. At doses of 350–400 μmol dm−3 myosmine induced apoptosis and
the hypoploid fraction. In vivo intraperitoneal injection of mice with 100 mg/kg of myosmine resulted in a
statistically significant increase in the percentage of cells in S phase; i.e. from 13.75 to 18.22 %. The percentage
of bone marrow cells in the G2/M phase increased from 6.79 to 8.93 % in treated mice compared
to controls. All of these results are in agreement with the hypothesis that myosmine possesses genotoxic
potential. (doi: 10.5562/cca1767)

Ključne riječi

myosmine, cytotoxicity, cell cycle, apoptosis, bone marrow

Hrčak ID:

74772

URI

https://hrcak.srce.hr/74772

Posjeta: 1.514 *