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https://doi.org/10.5562/cca1738

Separation and Quantification of 1,4-benzodiazepines: HPLC versus CZE

Květa Kalíková orcid id orcid.org/0000-0003-4988-8322 ; Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, 128 43 Prague 2, Albertov 2030, Czech Republic
Martina Riesová ; Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, 128 43 Prague 2, Albertov 2030, Czech Republic
Richard Chudoba ; Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, 128 43 Prague 2, Albertov 2030, Czech Republic
Martin G. Schmid ; Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Universitätsplatz 1, A-8010 Graz, Austria
Eva Tesařová ; Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, 128 43 Prague 2, Albertov 2030, Czech Republic


Puni tekst: engleski pdf 3.088 Kb

str. 367-373

preuzimanja: 2.087

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Sažetak

The goal of the present study was to separate a set of benzodiazepines, namely bromazepam,
oxazepam, nitrazepam, chlordiazepoxide, flunitrazepam, lormetazepam and diazepam by analytical scale
HPLC and CZE. The both methods for separation of these seven compounds from the 1,4-benzodiazepine
group were optimized and compared. LODs and LOQs were determined under the optimized conditions in
the both methods. The corresponding LOD and LOQ values are approximately three orders of magnitude
lower in HPLC than in CZE. As expected, elution order was found to be different for the both techniques.
As a result of a critical collation of all the parameters considered, RP-HPLC was found to be more suitable
for determination of the set of benzodiazepines. A real sample analysis was performed under optimized
conditions to demonstrate applicability of the proposed analytical methods. (doi: 10.5562/cca1738)

Ključne riječi

RP-HPLC, CZE, benzodiazepines, pharmaceuticals

Hrčak ID:

74775

URI

https://hrcak.srce.hr/74775

Posjeta: 2.673 *