Izvorni znanstveni članak

https://doi.org/10.5562/cca1795

Carbohydrate Composition of Endotoxins from R-type Isogenic Mutants of Shigella sonnei Studied by Capillary Electrophoresis and GC-MS

Annamária Bui ; Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs
Anikó Kilár ; Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary; Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Ifjúság útja 6., H-7624 Pécs,
Ágnes Dörnyei ; Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs; Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Ifjúság útja 6., H-7624 Pécs, Hungary
Viktória Poór ; Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs
Krisztina Kovács ; Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary
Béla Kocsis ; Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary
Ferenc Kilár orcid.org/0000-0001-5737-5514 ; Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs; Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Ifjúság útja 6., H-7624 Pécs, Hungary

Sažetak

The carbohydrate composition of the rough-type endotoxins (lipopolysaccharides, LPSs) from
Shigella sonnei mutant strains (Shigella sonnei phase II - 4303, 562H, R41 and 4350) was investigated by
capillary electrophoresis and GC-MS. The monosaccharides obtained by hydrolysis were determined by
capillary electrophoresis combined with laser induced fluorescence detection (CE-LIF) after labeling with
8-aminopyrene-1,3,6-trisulfonic acid (APTS) and by GC-MS as alditol-acetate derivatives. It was obtained
that the lipopolysaccharides of the isogenic rough mutants are formed in a step-like manner, containing
no heptose (4350), one D-glycero-D-mannoheptose (562H), or two or three L-glycero-Dmannoheptoses
(R41, 4303, respectively) in the deep core region. Besides the heptoses, the longest
LPS from the mutant Shigella sonnei 4303 contains hexoses, such as glucoses and galactoses, in a proportion
of approximately 3:2. This study provides a comprehensive comparison of the variability in
the carbohydrate part of the rough endotoxins extracted from Shigella sonnei isogenic mutants.
(doi: 10.5562/cca1795)

Ključne riječi

lipopolysaccharide structure; Shigella sonnei rough mutant; D-glycero-D-mannoheptose; capillary electrophoresis; CE-LIF; GC-MS

Hrčak ID:

74778

URI

https://hrcak.srce.hr/74778

Datum izdavanja:

13.11.2011.

Posjeta: 2.403 *